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The Research Of Peroxynitrite Generation Mechanism Of Overtraining Induced In Skeletal Muscle

Posted on:2015-02-07Degree:MasterType:Thesis
Country:ChinaCandidate:G X CuiFull Text:PDF
GTID:2297330431950632Subject:Physical Education and Training
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Overtraining is the body fatigue and function decreased caused by prolongedheavy load training, and can not be recovered in a short time. Overtraining is causedby a continuous exercise fatigue accumulation. When high-intensity exercise training,the body cells will produce oxidative stress. Exercise-induced skeletal muscle cells toproduce the most important free radical are superoxide anion(O2-)and nitric oxide(NO),and they can happen fast non-enzymatic chemical reaction to produceperoxynitrite (ONOO-). ONOO-is a stronger oxidant than O2-, can mediate enhancedoxidative stress, may be the key factor leading to exercise-induced fatigue.But theONOO-generating mechanism of excessive exercise-induced in skeletal muscle isunclear.Through injecting NADPH oxidase inhibitors to mice after Long-term swimtraining,we constructed experimental model. Whereby the article,we investigatedONOO-generating mechanism induced by overtraining in skeletal muscle.Research methods:64mice were randomly divided into6groups: control group(group C), Control+drug group (group CA), Swimming training (group T), Swimming training+druggroup (group TA), Overtraining group (group OT), Overtraining+drug group (groupOTA). The mice of group T were forced to swim training with non-weight-bearing,and the mice of group OT were loaded with5%of body weight tied to thetail.Training60minutes every day,6days every week for8weeks; For twoconsecutive days before sampling to inject NADPH oxidase inhibitorapocynin(10mg/kg body weight).Research results:(1) After8weeks of overtraining, Mice’s boad weight of training were lower thanthe control group, and the group OT decreased significantly in the eighth week; Hbconcentration decreased in the training group compared with control group(P<0.05);(2) Mice through eight weeks of swimming training,24hours after the lastmovement, NADPH oxidase’s activity in skeletal muscle compared with control groupincreased both in group T and OT (P <0.01),and OT increased more than group T (P<0.01);(3) Mice through8weeks of swimming training compared with control group:iNOS’s protein expression increased both in group T and TA (P <0.01),,iNOS andnNOS’s protein expression were all increased both in the group OT and OTA (P <0.01);(4) Mice through8weeks of swimming training,the skeletal of group OTgenerated excessive ONOO-compared with control group, ONOO-content increasedsignificantly (P <0.01),while the group OTA compared with the group T showed noincreased significantly.Conclusions:(1) Long-term endurance training increases skeletal muscle NADPH oxidaseactivity, and the higher the exercise intensity, the higher the increase in enzymeactivity.(2) Overtraining enhanced skeletal muscle iNOS, nNOS protein expression.(3) Overtraining induced NADPH oxidase reaction ROS in skeletal muscle, andthe latter with NO generated by NOS and/or nNOS catalytic reaction, leading togenerate excessive ONOO-.
Keywords/Search Tags:overtraining, reactive oxygen species, nitricoxide synthase, NADPHoxidase, peroxynitrite
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