Identification And Antibacterial Activity Of Deep-sea Sediment Culturable Bacteria In South Atlantic

On the earth, microorganism become high-profile in biological sciences withnumerous of advantages, such as large variety of species, tiny individual, extremelyhigh speed reproduction, strong metabolism and high probability genetic mutationsand so on. Deep sea has become a hot subject on account of its abundant biologicalresources and extreme environment. In addition, the analysis of the deep-seamicrobial diversity, not only helps us to understand the ecological environment ofthe deep sea and a better cognize of Marine resources, but also provided atheoretical basis for the development and utilization of marine microorganisms.In this researching, some isolates were selected to study the ability of anti thegrowth of Aspergillus parasiticus and Fusarium graminearum. At the same time, weobserve the bacteria colonial morphology and16S rDNA sequence analysis toidentify these isolates. In the end, all the samples that had been collected from theSouth Atlantic were selected to study their diversity.The different methods of Gas-bacilli confrontation and Oxford cup methodwere used to study the ability of anti the growth of Aspergillus parasiticus andFusarium graminearum. There are19.69%of the strains have a good effect on antithe growth of red material of Aspergillus parasiticus mutant NFRI-95and28.79%of the strains showed varying degrees of inhibition rate. There are18.18%of thestrains showed higher inhibition rate to Fusarium graminearum. In the Oxford cupstandoff method,17.82%of the strains have good red suppression effect on NFRI-95,10.3%of the strains of have good antibacterial effect on NFRI–95and15.88%of the strains have good antibacterial effect on Fusarium graminearum.243strains' identify results were gotten with16S rDNA sequence analysiswhich come from the separation of the early from the sediment samples. They arebelonged to3phylums and17genuses, namely Proteobacteria, Firmicutes andBacteroidetes. Bacillus has advantage on17genuses.683strains were selected to study their diversity which includ species diversity,genetic diversity and the analysis of the diversity of the function of antifungalstrains. Those strains include which440strains have been identified early and theyare come from15different samples of South Atlantic.24.5%of the strains areMarinobacter and22.8%of the strains are Halomonas, they are the advantage of27genus. Bacillus, Halomonas and Marinobacter were selected to study the geneticdiversity. Genetic matrix analysis method and making a phylogenetic tree were usedto do it. In the function of antifungal strains, most of them were Bacillus.