Reaserch Of SIRT2 In Lipid Metabolism

SIRT2 protein as a third category deacetylase family member is mainly localized in the cellular cytoplasm,which plays an important role in the regulation of the cell cycle,oxidative stress,lipid metabolism and neurodegenerative diseases.In which lipid metabolism,currently known SIRT2 can inhibit adipocyte differentiation through FOXO1,and can be used to modulate the expression of lipid metabolism-related genes through PGC-la in adipocytes.The ATGL is considered as a specific hydrolysis triglyceride lipase,but also as the rate-limiting enzyme in the process of degradation of lipid droplets.ATGL and hormone sensitive lipase(HSL)can be completed over 97%of the body lipid metabolism,it is also considered to be the mainly lipid metabolism enzymes.In this study,we firstly found an interaction between SIRT2 and ATGL by co-immunoprecipitation experiments,and a co-localization between this two proteins at the sub-cellular levels.And subsequently,we found a significantly enhanced ATGL's enzyme activity by SIRT2,and this effect is dependent on the deacetylation of ATGL by SIRT2.In addition,to explore the relationship between SIRT2 and the ATGL's activated factors CGI-58,we also found that the combination of CGI-58 and ATGL can enhances the interaction between SIRT2 and ATGL and promotes the enzymatic activity of ATGL.Moreover,The enhanced activation of ATGL by SIRT2 is in respondse to various external stimuli conditions.So,we found a new regulator for ATGL,which provides a guideline and reference for us to further study of lipid metabolism.