| Termites are social insects distributed widely in nature, usually distributed in tropi-cal and subtropical regions. According to the feeding diet, they can be divided into civil amphibious termites, soil-feeding termites and wood-feeding termites. As one of the few lower food chain insects that can digest lignocellulose,they play important roles in mate-rial cycel in nature.It was reported that intestinal symbiotic microbes play important roles in this process.The studies on the microbial resources in the gut of wood-feeding termites is significant for the further utilization of biomass energy and development new biofuel industry.The main results of this study are as follows:1.21 bacterial strains isolated from wood -feeding lower termites Reticulitermes chinensis Snyder that widely distributed in China were screened for their abilities to de-grade sodium carboxymethyl cellulose. The accuracy of these strains were identified by the analysis of their 16S rRNA genes. Congo red staining method was used to screen the cellulase activity.Eventually two strains, Streptomyces sp. and Bacillus sp. were con-firmed to have the activity of endoglucanase.2 Opitutaceae sp. TSB47 is a strain isolated from the intestine of Reticulitermes chinensis Snyder. The colony morphology is round, colorless, neat edges, moist, shiny, opaque. The cell size of it is about 0.8-1.0?m. It is gram -negative, non spore, flagel-la,without hair, no sporting. According to the 16S rRNA genes, this bacterium is phylo-genetically closest to Opitutaceae family Opitutus terrae PB90-lwith 93% sequence sim-ilarity. The growth temperature range is 20-35? and the growth pH range is pH6.0-8.5. It can use maltose, xylose, galactose as the carbon source. Phylogenetic analysis shows that Opitutaceae sp. TSB47 belongs to Opitutaceae family. The main amino acid com-ponents of bacterial cell wall are histidine, glycine, serine, alanin, and homoserine. |
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