Transcriptome Analysis Of Penicillium Strain HP7-1 And Its Cellulase-high-yield Mutation EU2106

This research uses the available material HP7-1,a Penicillium strain and EU2106,the cellulase-high-yield mutation of the former.We cultivate the conidiospores of both strain on Avicel cellulase inductive medium collect the mycelia to extract total RNA and made transcriptome analysis by taking advantage of Shenzhen Huada gene technology company.The results showed that those gene encoding cellulase such as CBH and EG of mutation are about there times higher in transcription level than those of wild type,which conforms to the enzyme activities performaced with DNS method.For one aspect,the transcriptome analysis confirm that cellulase activity of mutation rise derived from the rise of cellulase gene transcription.On the other hand,the transcritpome analysis showed that both wild type and mutation strain take the same sequence of cellulase encoding gene and key transcription factors such as cre1,ace1,hap2/3/5 and xyr1,which has different abundances in the two strain.Xyrl,the only one upregulated positive regulator,play a main factor in promoting cellulase activity.Finally,we find Unigene8063_All is a very important regulator via the analysis in GO annotation classification on different expression genes in more than two times abundance difference.Unigene8063_All,the blast result of which is FlbD a kind of conidiophore development protein,is two times less expressed in mutation than in wild type.Study of other filamentous fungi such as Aspergillus nidulans showed that the function of FlbD is promoting the filamentous fungi cell develop and differentiate by binding to the promoter of BrlA with other regulator such as FlbB.However,expressiong level of FlbB and BrlA in both strains is similar.The ultimate reason of higher cellulase expression in mutation is still unclear,more work need to be done.