The Study Of The Bacterial Diversity In The Gastrointestinal Tract Of Hipposideros Pratti And Hypsugo Alaschanicus

Bats are many zoonosis carriers of the pathogens that can cause a variety of zoonosis.Recent studies have been focusing on viruses in bats,but little has been focused on bacterial pathogens.Due to the development of tourism industry and the increasing of population density,people will be contact with bats more frequently,and the pathogens in bats could be more possibly transmitted to humans.So far,international studies of bats gastrointestinal bacteriawere rare,and it has not been reported at home.Given this,the stomach and intestinal bacteriaof Hipposideros pratti and Hypsugo alaschanicus were identified and analyzed in this study by the traditional isolation and cultivation,16 S rRNA sequencing technology and Illumina high-throughput sequencing technology.1.The results of the stomach and intestinal bacterial flora were separated by the traditional isolation and cultivation and 16 S rRNA sequencing analysis technology were as follows: A total of 8 isolates were identified from the stomach contents of Hipposideros pratti.These bacteria mainly belonged to Enterobacter,Pantoea,Morganella,Hafinia,Gibbsiella,LysinibacillusandLactococcal.A total of 10 isolates were identified from the intestinal content of Hipposideros pratti.These bacteria mainly belonged to Enterobacter,Citrobacter,Raoultella,Morganella,Providencia,Lysinibacillus,Enterococcus.A total of 7 isolates were identified from the stomach contents of Hypsugo alaschanicus.These bacteria mainly belonged to Klebsiella,Enterobacter,Aeromonas,Lactococcal,Morganella,Providencii,Klebsiella.Thirteen isolates were identified from the intestinal content of Hypsugo alaschanicus.These bacteria mainly belonged to Enterobacter,Klebsiella,Lactococcus,Staphylococcus,Vibrio,Proteus,Morganella,Providencia Escherichia,Shigella.2.The analyses of bacterial diversity in the gastrointestinal tract of H.pratti and H.alaschanicusby MiSeq high-throughput sequencing.The results were as follows: phylogenetic analysis results show that the gastrointestinal bacteria of Hipposideros pratti were mainly distributed in 6 Phyla: Proteobacteria,Firmicutes,Bacteroidetes,Tenericutes,Actinobacteria and Unclassified,dominated by Proteobacteria(56.4% in stomach and 46.0% in intestines)and Firmicutes(40.7% in stomach and 49.2% in intestines).There were 24 genera above 0.100% in stomach and intestine of Hipposideros pratti.Lactococcus and Hafnia were the 2 dominant bacterial genera in stomach accounting for 26.1% and 21.0% respectively.Enterococcus and Salmonella were 2 dominant genera in intestine accounting for 15.2% and 12.7% respectively.Phylogenetic analysis results show that the gastrointestinal bacteria of Hypsugo alaschanicus were mainly distributed in 5 Phyla: Actinobacteria,Bacteroidetes,Firmicutes,Proteobacteria and Unclassified.Proteobacteria and Firmicutes were the 2 dominant bacterial phyla in stomach accounting for 27.8% and 59.5%,respectively.Proteobacteria,Actinobacteria,Bacteroidetes and Firmicutes were 4 dominant genera in intestine accounting for 39.7%,15.7%,15.6% and 12.7% respectively.There were 20 genera above 0.100% in stomach and intestine Hypsugo alaschanicus.Lactococcus and Bacillus were the 2 dominant bacterial genera in stomach accounting for 46.1% and 7.4% respectively.Sphingomonas and Mycobacterium were 2 dominant genera in intestine accounting for 10.5% and 7.3% respectively.3.Most of the bacteria in the gastrointestinal tract of Hipposideros pratti and Hypsugo alaschanicus are human pathogens or conditional pathogenic bacteria,so we should avoid the pollution of water and food which caused by the living bats and their feces.The caves where Hipposideros pratti live should be prohibited from developing tourism,to prevent the transmission of diseases between humans and bats.