Secondary Metabolites From Deep Marine-derived Fungi, Aspergillus Sp. SCSIOW3, Cultivated With SBHA And 5-AC

The microbial species and number in nature is large, however, only about 5% of the total microbes in nature is known and used by mankind. For the marine and other extreme environments, due to the difficulty to obtain the samples, mankind couldn't make full use of these microbes.This paper focused on chemical diversity of fungi, including deep marine fungi, soil fungi and plant endophytes. Chemical epigenetic stimulation of these fungi was conducted, by adding small molecule chemical epigenetic irritant in the medium to activate the fungus metabolism. The stimulis including DNA methyltransferase inhibitor, namely 5-Azacytidine(5-AC), and histone deacetylase inhibitor, namely Suberohydroxamic acid(SBHA). After fermentation and extraction, we found that strain W3 had an apparent change in chemical profile after epigenetic stimulation by TLC and HPLC analysis.After ITS sequencing, W3 was determined as Aspergillus sp. by conparison with the database.A large scale fermentation of Aspergillus sp.SCSIOW3 was then conducted. We isolated seven compounds by using silica gel column, ODS C18 reversed-phase column, Sephadex LH-20 and HPLC. Sturctures were solved by means of spectroscopy analysis. Compounds 4 and 6 were new compounds, compounds 1(Diorcinol), 2(Sydonic acid), 3(3, 3'-Dihydroxy-5, 5'-dimethyldibenzofuran), 5(Cordyol) and 7(Cyclo-(L-Trp-L-Phe)) were known compounds. Compounds 1, 3, 4, 5 and 6 have the same diphenyl ether skeletal structure. The anti-inflammatory biological activity of the compounds 1, 2, 3, 5, 6, 7 were studied. Results showed that compounds 2, 3 5, 6, and 7 have strong inhibition activity against NO production, the IC50 was 7.19, 11.09, 5.38, 25.73, 22.36 ?M respectively. Compound 1 showed IC50 of 83.33 ?M.