Study On Dynamic Process Of The Interaction Between Plasma Active Substances And Cells In Culture Medium

Recently, the plasma medicine received a lot of attention in the plasma and biology community.Most of the plasma treatment targets are living tissues and cells immersed in liquids(such as blood or culture media). Reactive species generated by plasma in the gas phase are transported into the liquid and possibly converted to different types of reactive species, so that they can affect tissues or cells. Plasma can reach the internal cause irreversible damage to DNA, thereby killing the cells without destroying the cell morphology of cells.In this paper, through experiment and simulation, we analyzed the ability of the plasma on the inhibition of the cell proliferation and cell inactivation while having no effect on the cell morphology. Plasma was able to transmit to the liquid phase and cause irreversible damage to the cell while made no harm to the cell morphology. Take NO and OH as examples, we explained the concentration evolution process of the substance transmitted from gas phase to pure liquid phase by solving the rate equation. we further presented a numerical investigation of the interaction between the plasma and cells immersed inthe culture media. The space and time evolution of dissolved reactive species(ROS(O2-, OH, H2O2, HO2, O3) and RNS(O2NO-, NO, NO2-, NO3-, O2NOH)) are studied by the mass balance model in liquid. The reaction mechanism and effective transportation range of dissolved reactive species are analyzed.We also found that the p H of the liquid has significant effects on the concentration of O2-, HO2, O2NO- and O2 NOH in the culture media. The increasing concentration through cell membrane of HO2 and O2 NOH at acidic p H are consistent with higher bacterial inactivation efficiency of plasma treatment in acidic p H environment. The cellular and subcellular concentration of HO2, O2NO- and O2 NOH are studied by the conservation equations in a spherically symmetric concentration field. The plasma treatment increased the cellular concentration of O2NO- over 10 times. The HO2 generated by plasma aqueous species is the onl y way for superoxide to penetrate cell membrane and damage cytosolic fumarase B. This thesis conducted in-depth research into the whole chain of the plasma “gas phase-liquid phase-cell” transportation process, and discovered the main liquid active substances originated from the plasma and their interaction mechanism with the cells, which laid a solid theoretical foundation for further research.