The Roles Of As-gsk3? Gene Involved In The Early Embryo Development And Anti-stress Proccesses Of Artemia Sinica

Glycogen synthesis kinase 3?(GSK3?)is an ancient,multifunctional Ser/Thr protein kinase and belongs to CMGC family.GSK3? plays important roles in cell metabolism regulation,survival and embryonic development processes as an effector of PI3K/AKT and Wnt signal pathway.Artemia sinica undergoes an embryo diapause process when they encounter high salinity or low temperature stress and diapause can be terminated in certain conditions.The functions of GSK3? in A.sinica diapause termination and embryonic development still remain unknown.In present study,the full-length of As-gsk3? cDNA was1801 bp,encoding a 422 amino acid protein.The expression locations of As-gsk3? were observed throughout the whole body by in situ hybridization.Although the result of real-time PCR showed a significant increase in 3 d,As-GSK3? appeared no obvious change by western blot.Remarkably,the As-GSK3?-Ser9 p expression level had a significant difference during embryo development stages.This suggested As-GSK3? takes part in embryo development by phosphorylation regulation.The change of As-TREH expression pattern may correspond to trehalose content and cause glycogen synthesis change in embryonic development process.The expression downregulation of As-GSK3?-Ser9 p,As-GSK3?-Ser21 p and As-TREH may contribute to survive in the low temperature or high salinity challenge.Furthermore,the increasing As-GSK3?-Ser9 p expression level may result in pro-survival mechanism starting at5 °C.In conclusion,the expression pattern results of related As-GSK3? and As-TREH in early embryonic development and low temperature or high salinity stress indicated that these proteins may play important roles in glycogen metabolism regulation and anti-stress as well as embryonic restarting development processes.