CSN Complex Subunit PfCsnE Regulates Secondary Metabolism And Developent In Pestalotiopsis Fici

CSN complex (COP9 signalosome), is a highly conserved multiprotein complex in all eukaryotes and involved in the regulation of multiple important biological processes. Here we uncover the influence of CsnE, the fifth subunit of CSN on the development and secondary metabolism in P. fici, The main results were as follows:First of all, a homology search of the P. fici genome with CsnE identified PfCsnE with bioinformatics analysis. PfcsnE gene deletion mutant (?PfcsnE) that stopped conidial formation, PfcsnE complemented strain (?PfcsnEC) restored phenotype and in accordance with the wild type (WT). This indicates that PfCsnE is essential for the formation of conidia.Secondly, analysis the SMs of ?PfcsnE mutant, secondary metabolite profile changed very obviously. 6 new compounds were identified. Furthermore in comparison to WT, the production of chloroisosulochrin was significantly increased, while ficiolide A production decreased obviously in the ?PfcsnE strain.Finally, transcriptome analysis of WT and APfcsnE strains indicated that PfcsnE impacts the expression levels of 11.91% genes of P.frci genome, including genes involved conidial formation and morphology.PfCsnE affected the expression of 12 biosynthetic gene clusters (BGCs),9 were up-regulated and 3 were down-regulated.In conclusion, this study clarified the regulatory of CsnE on the development and secondary metabolites in P. fici and provided new pathway to discover new compounds.