Construction Of Poligd Deletion Mutant For Efficient Gene Targrtiong And Functional Analysis Of PoVta1p In Protein Transport In Penicillium Oxallicum

Recently,the biofuel industry discovered that filamentous fungus Penicillium oxalicum is a potential alternative to Trichoderma reesei,since P.oxalicum strains can secrete more balanced native lignocellulolytic enzyme systems and display higher?-glucosidase activity compared to T.reesei.The ESCRT(endosomal sorting complex required for transport)gene VTA1 in eukaryotes serves important functions in secretory pathway.However,little is known about the functions of the Vta1 protein in filamentous fungi.In this study,the non-homologous end-joining(NHEJ)gene ligD deletion mutant ?PoligD was constructed which is a suitable recipient for highly efficient gene targeting experiments in P.oxalicum.Furthermore,the PoVTA1 gene from P.oxalicum HP7-1 was cloned and functional analysis.The PoVtalp participates in important morphogenetic events,including sporulation and hyphal growth.The PoVtalp is location in cytoplasm of P.oxalicum.Furthermore,deletion of the PoVTA1 gene significantly increased the activities of amylase(up to 1.9-fold),celluloase and xylanase.The results suggested that the PoVtalp mediates protein transport,particularly that of xylanase,celluloase and amylase.Thus,this study enhances our knowledge of the underpinnings and mechanisms associated with the secretion of cellulase,xylanase and amylase by filamentous fungi,which can be further manipulated in industrial settings to enable increased protein production.