Study On Optogenetics Regulation Of Pseudomonas Aeruginosa By Blue Light Sensor System

In recent years, with the continuous development of synthetic biology, a variety of novel biological technology also came into being. Among them, the optogenetics technology has become a vigorous development of the field,through the construction of engineering photoreceptors, to control the life of living cells and organisms. We selected the chromosome gene integration system mini-Tn7 to integrate the blue light response system into Pseudomonas aeruginosa strain PAO1. Integrating genes into chromosomes has a greater stability in bacteria than plasmid cloning systems because no similar plasmid loss occurs. In addition, the mini-Tn7 system has a higher conversion rate and a specific binding site than any other transposon subsystem, and does not occur in any position inserted into the genome. The pDawn-Tn7 light control system, which is mentioned in this paper, provides a strong support for the expression of space-time control genes in Pseudomonas aeruginosa, and can also meet the requirements of precise control of the biological behavior of Pseudomonas aeruginosa.By introducing the reporter gene sfGFP green fluorescent protein into the light control system, the expression of the light-induced gene of the blue photoreceptor in Pseudomonas aeruginosa was observed. Many persistent bacterial infections are caused by biofilms. A typical example is a devastating pulmonary infection caused by Pseudomonas aeruginosa in patients with hereditary disease cystic fibrosis, once Pseudomonas aeruginosa colonizes the cystic fibrosis lungs, forming a biofilm, even the most Invasive antibiotic therapy cannot eradicate all bacteria. In this study, the introduction of PA2133 in the control system, the functional protein, can inhibit the formation of Pseudomonas aeruginosa biofilm. This is because the PA2133 gene contains the EAL domain, which expresses the protein equivalent to phosphodiesterase,phosphodiesterase can degrade c-di-GMP, c-di-GMP stimulates the biofilm in the viscous matter and Extracellular polymer biosynthesis, and degradation caused by bacterial separation, so PA2133 overexpression can cause biofilm degradation. Thus,this paper provides a new idea for the prevention and control of biofilm.