Apobec-1 Complementation Factor (A1Cf) Inhibits Epithelial-mesenchymal Transition And Migration Of Normal Rat Kidney Proximal Tubular Epithelial Cells

Objective To explore the spatio-temporal expression specificity in C57BL/6 mouse kidney and the influence of A1 CF in epithelial-mesenchymal transition(EMT)process of rat kidney tubular epithelial cells NRK52 e.Methods 1.C57BL/6 mouse kidney of E11.5d,E12.5d and E15.5d were selected for whole mount in situ hybridization,and the kidney of adult C57BL/6 mouse were separated into section for in situ hybridization to explore A1 CF spatio-temporal expression specificity.2.The two variants of A1CF(A1CF/A1CF(-8AA))were transiently transfected into NRK52 e cells,the epithelial marker E-cadherin and the mesenchymal markers vimentin and ?-smooth muscle actin(?-SMA)were detected by western-blot and immunofluorescence staining assays and effects of A1 CF on cells migration were investigated by scratch wound healing assay.3.A1CF-siRNA were transiently transfected into NRK52 e cells,the EMT-related markers were measured by western-blot and immunofluorescence staining assays and effects of A1 CF on cells migration were investigated by scratch wound healing assay.Results 1.In C57BL/6 mice,A1 CF was weakly expressed in embryonic kidneys from E15.5dpc while strongly expressed in mature kidneys after birth,and it mainly existed in the tubules of inner cortex in adult mouse kidney.2.Ectopic expression of A1 CF up-regulated the epithelial marker E-cadherin,and down-regulated the mesenchymal markers vimentin and ?-SMA in NRK52 e cells,and inhibited NRK52 e cells migration.Notably,the two A1 CF variants led to the similar trend in the EMT process.3.Knockdown of A1 CF down-regulated the epithelial marker E-cadherin,and up-regulated the mesenchymal markers vimentin and ?-SMA in NRK52 e cells,and enhanced NRK52 e cells migration.Conclusion Our data suggest A1 CF was weakly expressed in embryonic kidneys from E15.5dpc while strongly expressed in mature kidneys after birth,and it mainly existed in the tubules of inner cortex in adult mouse kidney.Besides,A1 CF may be an antagonistic factor to the EMT process of kidney tubular epithelial cells.