A Study On The Culture Conditions For High-yield Of Astaxanthin From Haematococcus Pluvialis

In order to obtain the conditions of high density cultivation and induction of high astaxanthin productivity of Haematococcus pluvialis,the culturing and inducing conditions were observed through single-factor experiments.The optimal basic culture media were determined,firstly.And then three plant growth regulators,indole-butyric acid(3-IBA),naphthyl acetic acid(NAA)and gibberellin(GA3),and three low toxicity herbicide S-Metolachlor,Butachlor and MCPA-Na,were applied to test their potential effect on cell growth and astaxanthin production in the green-cell stage and astaxanthin-induction stage,respectively.The results are as follows:(1)The modified BG-11 medium,24-26°C,Na HCO3 1.0g/L were the optimal conditions for H.pluvialis cell growth,and the maximum cell concentration reached to 160×104 cell/m L at the those conditions.(2)Plant regulators,3-IBA,NAA and GA3,were added into the cultivation media at different concentration to determine the effect on the algal growth and astaxanthin production.For 3-IBA,10 ?g/m L in green-cell stage and 25 ?g/m L in astaxanthin-induction stage were the optimal comditions for cell growth and antaxanthin production,and the maximum cell concentration and astaxanthin content reached 153×104 cell/m L and 13.77 mg/L at these concentrations;For NAA,the optimal conditions were 5?g/m L in green-cell stage and 25?g/m L in astaxanthin-induction stage,and the maximum cell concentration and astaxanthin content reached 140×104 cell/m L and 13.91 mg/L at these conditions;For GA3 5 ?g/m L in green-cell stage and 25 ?g/m L in astaxanthin-induction stages were the optimal concentration conditions for cell growth and astaxanthin accumulation,and the maximum cell concentration and astaxanthin content reached 115×104 cell/m L and 12.75 mg/L at these conditions.(3)Several low toxicity herbicide,S-Metolachlor,Butachlor and MCPA-Na,were added into the cultivation media at different concentration to determine the effect on the algal growth and astaxanthin production.For S-Metolachlor,2 ?g/m L in green-cell stage and 5 ?g/m L in induction stage were the optimal concentration for cell growth and astaxanthin accumulation,and the maximum cell concentration and astaxanthin content reached 194×104 cell/m L and 15.99 mg/L at these conditions.As for Butachlor,no catalytic role were observed in the study,the cells growth were inhibited at low concentration.For MCPA-Na,3 ?g/m L in green-cell stage and 5 ?g/m Lin induction stage were the optimal concentration for cell growth and astaxanthin accumulation,and the maximum cell concentration and astaxanthin content reached 190×104 cell/m L and 12.79 mg/L at these conditions.