Regulation Of Cyclooxygenase-2 Endoplasmic Reticulum Autophagy Of DF-1 Cells Induced By Cr(Ⅵ)

Endoplasmic reticulum stress and autophagy are adaptive responses to cell damage,which have certain protective effects on intracellular homeostasis,but excessive endoplasmic reticulum stress and autophagy may cause damage to cells,and the serious cause of cell death.Six valent chromium is a common environmental pollutant.Its exposure can lead to cell autophagy,and how to mitigate the autophagy effect caused by six valent chromium needs further exploration.Some studies have shown that cyclooxygenase 2(COX-2)can be induced by some heavy metals to produce endoplasmic reticulum stress and autophagy.Therefore,there may be some intrinsic relationship between six valence chromium,COX-2,endoplasmic reticulum stress and autophagy.This study explored the role of COX-2 in the autophagy of DF-1 endoplasmic reticulum induced by Cr(VI).In this experiment,hexavalent chromium is used as an extraneous substance to explore the changes of endoplasmic reticulum stress,autophagy and COX-2 level,and to determine the relationship between the three.This study provides evidence that Cr(VI)can induce endoplasmic reticulum stress and autophagy in DF-1 cells.It is also found that autophagy induced by Cr(VI)is related to the high expression of COX-2 in DF-1 cells.Further studies show that COX-2 inhibitor,NS-398,can reduce cell death caused by Cr(VI)induced injury and autophagy.In addition,ER stress in Cr(VI)treatment was proved to be a requirement for over expression of COX-2 in cultured cells.Here,this experiment provides the innovation of Cr(VI)induced autophagy in DF-1 cells.So far,this study is the first to report the role of six valent chromium in the high expression of COX-2 in DF-1 cells,which regulates autophagy.