The Analysis Of Components Of Yeast2.614's Pine Polyphenols Derivatives And It's Physiologically Active Research On Diabetes Mice

Biotransformation technology could be used to synthesize some material withspecail structure, induce modification of natural products and improve the activity ofsome specific substance. Some polyphenols will become polyphenol derivatives withstronger physiological activity after biotransformation which is proved by some paper,but there has no paper about the biotransformation of pine polyphenol. This article willtake pine polyphenols which enjoy the activity of antioxidant, hypoglycemic,hypolipidemic as raw materials, study systematically on structure and physiologicalactivity of pine polyphenol derivatives synthesized with pine polyphenol bybiotransformation.9different types of yeasts are chosen as microbes forbiotransformation to do research on antioxidant, hypoglycemic and hypolipidemicactivity of9types of pine polyphenol derivatives generated by biotransformation. Then,this article will get the best pine polyphenol derivative and yeast for biotransformation.The infrared spectrometer and liquid mass spectrometer will be used to analyze themain components of the pine polyphenol derivative with the strongest physiologicalactivity. Finally, this article study on the mechanism of action of the polyphenolderivative's best physiological activity through animal experiment. The main researchcontent can be listed as:(1) Choosing9yeasts for polyphenols' biotransformation, and getting9relevantderivatives, then, analyzing the antioxidant, hypoglycemic and hypolipidemic activityof these9pine polyphenols derivatives. The antioxidant activity was analysied byreducing capacity, hydroxyl radical scavenging ability and ABTS·+free radicalscavenging capacity; To hypoglycemic, the main evaluation was its inhibitory effect onthe activity of ?-glucosidase. To Hypolipidemic, cholate binding capacity in vitro wasthe key measuring index. The result shows that, there are8pine polyphenol derivativesenjoying higher hypoglycemic ability, and pine polyphenol derivatives got by2.614yeast has the highest hypoglycemic ability; Compered with pine polyphenol, all of the9pine polyphenol derivatives have higher hydroxyl radical scavenging ability, and2.614yeast pine polyphenol derivatives is the best; Only2.614yeast pine polyphenolderivatives has a stronger reducing capacity; All of the9pine polyphenol derivativeshave lower ABTS·+free radical scavenging capacity. Among9pine polyphenolderivatives,2.614yeast pine polyphenol derivatives has a higher hypolipidemic activity.Above all, we get the best yeast,2.614yeast, for biotransformation, and its' pine polyphenol derivatives enjoy the best physiological activity.(2) Using infrared spectroscopy and high performance liquid chromatography-massspectrometry to analyze the polyphenol derivatives generated by2.614yeast throughbiotransformation.2.614yeast pine polyphenol derivatives is more complex thanpolyphenols; Pine polyphenols and2.614yeast polyphenol derivatives enjoy somecommon materials, they may be: dimethyl delphinidin-3-O-arabinoside, rhizomakaempferiae phenol-3-O-buckthorn indican, clove flavin and amentoflavone; The2.614yeast pine polyphenol derivatives may have some derivatives of clove flavin andpuerarin. They prove that2.614yeast has bioconverse polyphenols to new2.614yeastpine polyphenol derivatives.(3) By four the alloxan, diabetic mouse model was established. Through gavaging2.614yeast pine polyphenol derivatives and analyzing body condition, diet, blood sugar,antioxidant and lipid analysis for diabetic mice given the medicine, it can be kown that2.614yeast pine polyphenol derivatives has symptom relief on diabetic mice for their "a little as san-duo ", improves the tolerance of diabetic mice sugar and reduces fastingblood glucose value. Except the recovery of SOD activity,2.614yeast pine polyphenolderivatives perform a better effect on antioxidant and lipid indexes. This shows that2.614yeast polyphenol derivatives may alleviate the mice diabetic disease byimproving antioxidant enzymes, reducing lipid peroxidation, reducing blood fat.Above all, the2.614yeast could produce pine polyphenol derivatives with strongerhypoglycemic activity through biotransformation. The polyphenol derivatives couldshow the hypoglycemic fuction through improving diabetic mice antioxidant enzymes,reducing lipid peroxidation and reducing blood fat. This article can contribute to thedevelopment of hypoglycemic natural drug and the biological modification research ofnatural products.