Biological Effects Of 4-BDE And BaP On Seedlings Of Triarrhena Sacchariflora And Salix Matsudana F. Pendula

The pollution level, migration, transformation, toxicology and degradation of POPs have attracted highly attention in the world. 4-BDE was the low brominated diphenyl ether, and existed extensively within ambient medium such as air, water, sediment, aqutic organism and so on. 4-BDE was a representative of mono-brominate diphenyl ethers. Ba P was the most toxic pentacyclic aromatic hydrocarbon with enrichment, high toxicity and volatility, and considered to be the main influencing factor of cancer. 4-BDE and Ba P could be enriched and concentrated through the food chain, and had potential harm to human and ecological environment. Phytoremediation could minimize the damage to environment. Phytoremediation was an important aspect of bioremediation, and had become the hot shot in the world. Plants response and related mechamisms of POPs exposure are the basis of the mechanism of phytoremediantion.In this paper, Zea mays, Arabidopsis thaliana, Triarrhena sacchariflora and Salix matsudana f. pendula were used to explore the responses to the 7 kinds of POPs exposure. The main results are as follows:(1) Within the designed exposure dose range of 0 mg/L-20 mg/L, the coleoptile elongation test results showed that higher concentrations of the classical IAA, 2,4-D, aroclor1254, PCB 126, PCB 28, BDE-209 and Ba P were inhibitory on elongation growth, and lower concentrations could promote the growth, resulting in bell-shaped dose-response curves. But aroclor1242 and 4-BDE had no significant influence on the growth of maize coleoptile.(2) Within the designed exposure dose range of 0 mg/L-20 mg/L, aroclor1254, aroclor1242, PCB 28 and BDE-209 could induced the DR5::GUS gene stable response in Arabidopsis thaliana, but PCB 126, 4-BDE and Ba P could not induce the gene expression.(3) 4-BDE exposure alone and hormone collaborative exposure could affect growth and development of silvergrass and willow. The maximal effective dose of 4-BDE exposure was 3 mg/L. 4-BDE exposure could shorten adventitious roots differentiation time of silvergrass, and increase the biomass of silvergrass. The effect of 4-BDE synergistic hormone was higher than 4-BDE exposure alone.4-BDE exposure alone could prolong adventitious root induction of willow, and could increase the biomass. 4-BDE synergistic hormone exposure could inhibit adventitious root induction, be same with positive control of stem-leaf biomass.(4) Ba P exposure alone and hormone collaborative exposure could affect growth and development of silvergrass and willow. The maximal effective dose of Ba P exposure was 4 mg/L. Ba P exposure could shorten adventitious root differentiation time of silvergrass. Ba P synergistic hormone exposure could increase the biomass of silvergrass, Ba P exposure alone could increase the biomass of adventitious root, be same with positive control of stem-leaf biomass.Ba P exposure alone could shorten adventitious root differentiation time of willow, increase the biomass of adventitious root, and increase the biomass of stem-leaf biomass and be same with positive control. Ba P synergistic hormone exposure could inhibit adventitious root induction, be same with positive control of stem-leaf biomass.(5) The levels of auxin and cytokinin could significantly influence by 4-BDE and Ba P exposure. 4-BDE in media of 1/2MS caused significant changes on the endogenous IAA?ZR?DHZR and i PA contents of silvergrass, the endogenous IAA/ZR and IAA/DHZR higher than the blank control. 4-BDE added in media of 1/2MS+0.5 mg/L NAA+0.5 mg/L IBA significantly affected resulted in the endogenous IAA?ZR?DHZR and i PA contents of silvergrass, and the endogenous IAA to cytokinin ratio was near the same with the positive control.Ba P in media of 1/2MS did not significant affect the endogenous IAA?ZR?DHZR and i PA contents of silvergrass, but the endogenous IAA/DHZR higher than the blank control. Ba P added in media of 1/2MS+0.5 mg/L NAA+0.5 mg/L IBA resulted insignificant effects on the endogenous IAA?ZR?DHZR and i PA contents of silvergrass, and the endogenous IAA level was higher than cytokinin, the endogenous IAA to cytokinin ratio was higher than the positive control.The biological effects of maize coleoptiles, DR5::GUS responses, effects on the adventitious roots differentiation of Triarrhena sacchariflora and Salix matsudana f. pendula and endogenous hormone levels at the early stage of adventitious roots differentiation indicated that 4-BDE could influence plant organs differentiation and growth by affecting endogenous auxin and cytokinin. The effect of Ba P could have other mechanisms besides the influence of the endogenous hormone, which needed to be further studied.