Hydrogen Producing Microorganisms In Different PH Condition Analysis Of The Differences In Protein Function

Dark anaerobic fermentation hydrogen-production technology is an efficient microbial hydrogen-production technology with high efficiency and stable hydrogen-production effect. It is also easy to industrialized production etc., and this method combines wastewater treatment with production of hydrogen. Therefore studies of anaerobic fermentation microorganisms have great significance.When the temperature is controlled at 35 ?, and five different p H gradient conditions were set:p H 5.5, p H 6.0, p H 6.5, p H 7.0, p H 7.5, the hydrogen-producing bacteria were cultured to maintain a constant p H value by a microbial fermentation tank with the automatic control system of p H value, the affecting the performance of hydrogen-producing bacteria is researched in the batch cul of microbial fermentation tank in different constant p H conditions, and the changing rule of biomass and residual glucose concentration are analyzed in different time the protein identification of hydrogen-producing bacteria is studied, hydrogen-production rate of the maximum period under the condition of p H5.5 and p H6.5. differentially expressed proteins are retrievalled Data and contrast analyzed to obtain the function protein information of microorganisms producing-hydrogen metabolism, and the possibility mechanism of action is analyzed by the differentially expressed proteins in the metabolism of microorganisms producing hydrogen.Results of research about microbial fermentation tank of hydrogen production at constant p H value control show that, under the condition of p H value of 5.5, the accumulative gas production of microbial fermentation is 5940 ml/L, the cumulative amount of hydrogen production is 1872 ml/L, the hydrogen content is 31.5%. Under the condition of p H value of 6.5, the cumulative amount of hydrogen production is 3412 ml/L, the cumulative amount of hydrogen production is the highest, hydrogen content is 33.3%. At the same time, the residual reducing sugar concentration in fermentation liquor reduce from 22 g/L to 3.52 g/L, the utilization rate of reducing sugar is as high as 84%, the strains of the optimum hydrogen-production p H condition is p H 6.5. Under the condition of p H value for p H7.5, The accumulative gas production is 4829 ml/L, the cumulative amount of hydrogen production is 1046 ml/L, the hydrogen content is 21.6%.The production-hydrogen microbial differences in protein expression spectra take by Using two-dimensional electrophoresis technology at the p H5.5 and p H6.5 two groups of different constant p H conditions when the optimal performance of hydrogen-producing. We use the Image the Master 6.0 application software to statistical analysis of data, and statistic two groups of experiment repeated point matching, the differences of protein points are comparatively analyzed though the matching expression abundance changes, we win the 63 significant changing of differentially expressed proteins, there are 34 raised the differentially expressed proteins and 29 differentially expressed proteins. The protein points are identificated by the mass spectrum, The Type and name of differences in protein are determined through retrievalling such as NCBI database. The 29 differentially expressed proteins are retrievalled, including 21 increase expression of differentially expressed proteins and 8 kinds of expression of differentially expressed proteins. According to the results of protein identification, each protein is retrievalled in literature system, and proteins are classified in function according to the statistical data: There are mainly 15 proteins related to the gene encoding protein; there are three kinds of protein related to the glycolysis; there are two kinds of proteins associated with cell division; the metabolism of hydrogen producing bacteria to production-hydrogen way protein has two kinds: pyruvate flavin oxidoreductase protein and riboflavin synthase. Pyruvate flavin oxidoreductase directly to attend Catalytic reaction of pyruvate reduction way in the microorganisms producing hydrogen metabolism of anaerobic fermentation process, there is a clear and effective producing-hydrogen acceleration, the effecttion is remarkable. And riboflavin synthase synthesize riboflavin(dehydrogenase) by indirect way, the increasing of riboflavin synthase expression quantity may also is one of the factors of the amount of hydrogen production increasing. The important hydrogen protein identification and function analysis in the process of producing hydrogen microbial production provides an important basis for the mechanism of microorganisms producing hydrogen research.