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Construction Of Surface Enhanced Raman Spectroscopy Detection Platform Based On Functional Nanocomposites And Its Application

Posted on:2016-04-21Degree:MasterType:Thesis
Country:ChinaCandidate:J H BaiFull Text:PDF
GTID:2311330473466417Subject:Analytical Chemistry
Abstract/Summary:PDF Full Text Request
Functional nanoparticals were extensively applied to bio-labeling and chemical analisis for its unique physical and chemistry properties. Meanwhile, surface enhanced Raman analysis methods were famous for its high sensitivity and optical stability. Combined functional nanoparticals and Surface Enhanced Raman Spectroscopy together, it was capable to detect target with high sensitivity. Based on these advantages, combined functional nanoparticals and SERS means together, we achieved to detect target with high sensitivity and selectivity. The contents of the experiments are as followed:(1)Surface Enhanced Raman Spectroscopy amplified assay based on SWNT/DNA@Ag NPs nanocomposites. In this exeperiment, we took advantages of single walled carbon nanotubes'(SWNTs) distinctive Raman scattering features, ss DNA were absorbed on the surface of SWNTs through electrostatic interaction, after which Ag NPs were in suit growthed on the surface of SWNTs. Experimental results showed that there was a linear relationship between the Raman intensity and the concertration of ss DNA in the solution. Inspired by this, the SWNT@Ag NPs hybrid nanocomposite was employed to realize d micro RNA detection. This system is also sensitive to crude extractions from human breast cancer cell and even patient tissues before and after chemotherapy with high sensitivity and stability.(2)Micro RNA ultrasensitive detection based on ion-inducing aggregation and Hybridization Chain Reaction. Ion-inducing aggregation is the one of the most common methods for Surface Enhanced Raman Spectroscopy. In this exeperiment, we take the advantage of ion-inducing aggregation cascade amplification caused by Ag+ and introduced Hybridization Chain Reaction. A new sensing approach for selective and ultrasensitive quantitative detection of mi RNA based on HCR and SERS methods has been developed. The strategy has low background signal and universality.
Keywords/Search Tags:Functional nanoparticals, Surface Enhance Raman Spectroscopy, Single Walled Carbon Nanotubes, micro RNA, Hybridization Chain Reaction
PDF Full Text Request
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