Study On The Preparation And Protein Adsorption Properies Of ZrO₂/PVDF Hybrid Membrane

Zirconium oxide (ZrO2) has attracted much more attention for its good biocompatibility, huge surface, structural stability, low cost and non-toxicity. The surface hydroxyl group of ZrO2 Particles can be used to adjust the surface electricity, which is helpful for the surface functionalization. Taking full advantage of electristatic interaction and bonding functional groups, proteins can be separated from the mixture by adsorption. The PVDF/ZrO2 Hybrid Membranes combine good adsorption activity of ZrO2 nanoparticles and excellent seperation performance of PVDF, which might possess good adsorption and seperation properties toward proteins.In this paper, ZrO2 nanoparticles were prepared by hydrothermal method, and 3-ammonium propyl triethoxy silane (APTES) was used to modify the surface of ZrO2. Some means, such as X-ray diffraction analysis (XRD), laser particle size analyzer, Zeta potential measurement and Infrared spectrum (FT-IR), were used to character the samples. The bovine serum albumin (BSA) and Hemoglobin (Hb) were used as a target substance to study the adsorption properties of the samples. The results show that BSA, as a acidic protein with rich exposed surface carboxyl, combined with APTES-ZrO2 via amido bond. The optimum adsorption conditions were an follows:150?g/mL, pH=5, t=12 h, under these conditions the maximum adsorption capacity of hybrid membrane was 117.3 mg/g. The adsorption dynamics model met better with the pseudo-first-order adsorption model. Adsorption isotherm met better with the Langmuir model. Hb, as a neutral, combined with APTES-ZrO2 via electrostatic interaction and hydrogen boneing. The optimum adsorption conditions were an follows:150?g/mL, pH=5,t=60 min, under these conditions the maximum adsorption capacity of hybrid membrane was 120.0 mg/g. The adsorption dynamics model met better with the pseudo-first-order adsorption model. Adsorption isotherm met better with the Langmuir model.The APTES-ZrO2/PVDF hybrid membranes were successfully prepared using physical blend process. The morphology, thermal stability and ZrO2 loading percent were characterized by scanning electron microscope (SEM), TG-DTA analysis, X-ray diffraction analysis (XRD) and infrared Spectrum (IR), respectively. The hemoglobin (Hb) was used as absorption farget, the adsorption ability of PVDF and APTES-ZrO2/PVDF hybrid membranes were studied under different condition. The results show that APTES-ZrO2 was successfully deposited on the PVDF film. The pore size of hybrid membranes aperture increases with ZrO2 addition, and the thermal stability of hybrid membranes improved too. The optimum adsorption conditions were as follows:150 ?g/mL, pH=7, t=45min, under these conditions the maximum adsorption capacity of hybrid membrane was 0.19 mg/cm2. The adsorption dynamics model met better with the primary adsorption model. Adsorption isotherm met better with the Langmuir model.