Synthesis Of Fluorescent Chemosensors Based On A Strategy Of Rhodamine And Its Application In The Detection Of NADH

Fluorescence detection in now is a kind of cells commonly used means,Because of its convenient and quick,low cost,and has high sensitivity and specificity.So compared with the traditional detection method,fluorescence analysis is more popular.Known as Rhodaminebased on oxygen mixed anthracene fluorophore matrix structure have a very good fluorescence properties,xanthene,the nature of the derivatives,such as energy can control good adaptability to pH,a longer wavelength at about 500 nm emission,which in the visible light region,more suitable for clinical examination.In cell metabolism,NAD+ / NADH is a core of metabolites,is the best parameters for characterization of cell metabolism imbalance,and cancer cells have different levels of NADH.NAD+ / NADH is a pair of electrons in a cell carrier,is responsible for transport electrons in the redox reaction,when the intracellular redox reactions,most of the time have the participation of NAD+ / NADH,so design can real-time detection level of NADH/NAD + in living cells is necessary,has very important strategic significance as well as to the future cell organism metabolism level,the level of cell growth,cell pathological changes,cell aging,cancer research has important value.We have synthesized 0902A、0902B1、0902B2、0902C,Has a great deal of conjugate structure,and by 1 HNMR,ms and their characterization.These compounds have spiral lactone ring structure,when combined with NADH,diethylamine base group in molecular ends in N,of the electrons of the atoms as electron acceptor accept from NADH to redox reaction,oxidation of NADH into NAD+,N atom due to accept electronic after have stronger for electronic ability,thus to oxygen mixed anthracene transmission electron structure of the fluorescence emission group.Fluorescent groups stimulated,diethylamine with electronic ability of N atoms make the highest occupied orbital electrons into excited oxygen mixed structure of anthracene fluorophore vacated by an electron electronic track,make oxygen mixed anthracene fluorophore electrons are unable to return to the original ground state by the light rail emission fluorescence,cause oxygen mixed fluorescent anthracene groups fluorescence quenching,it is a typical ICT mechanism.These compounds have spiral lactone ring structure,when combined with NADH,diethylamine base group in molecular ends in N,of the electrons of the atoms as electron acceptor accept from NADH to redox reaction,oxidation of NADH into NAD+,N atom due to accept electronic after have stronger for electronic ability,thus to oxygen mixed anthracene transmission electron structure of the fluorescence emission group.Fluorescent groups stimulated,diethylamine with electronic ability of N atoms make the highest occupied orbital electrons into excited oxygen mixed structure of anthracene fluorophore vacated by an electron electronic track,make oxygen mixed anthracene fluorophore electrons are unable to return to the original ground state by the light rail emission fluorescence,cause oxygen mixed fluorescent anthracene groups fluorescence quenching,it is a typical ICT mechanism.Through screening system we finally determined under water and ethanol in different ratio of system(the proportion of each compound are different),under such a system of NADH has high selectivity,sensitivity,and other small molecular ion without interference.On this basis we by adjusting the pH value of the right,select the appropriate solvent system,can make0902A、0902B1、0902B2、0902C for NADH has the very high selectivity.