Study On The Preparation Of A Micro-nano Mytilus Edulis Powder Based Preservative Agent And Its Preservation Effect On Hairtail

Hairtail has a high nutrient content of protein and fat. However, most of it is from distance fishing and long-distance transport is required. Therefore, It is not easy to save.Nano-shell powder with a bactericidal effect has drawn attention from both at home and abroad in recent years. This paper studies the development of a preservative which based on micro-nano mytilus edulis shell powder and its preservation performance of hairtail.There are four sections in this paper: The first is determination of the basic ingredients of hairtail and characterization of micro-nano mytilus eduli shell powder. The second is the optimization of micro-nano mytilus eduli shell powder's preservative ingredient; the third is the study of micro-nano mytilus eduli shell powder's preservative on preservation of hairtail; the last is the study of micro-nano mytilus eduli shell powder's preservative on mechanism of hairtail. The main obtained results are as follows:1.The basic ingredients of hairtail is: moisture content 71.10 ± 0.09%, fat content13.72 ± 0.40%, crude protein content 12.37 ± 0.20%, ash content 1.52 ± 0.23%. Based on the characterization of IR, BET and TGA of micro-nano shell powder, the main ingredients is calcium oxide, and to the gas and liquid small molecules with more stable adsorption.2.Micro-nano mytilus eduli shell powder's preservative includes micro-nano shell powder, sodium alga acid and CMC. Orthogonal optimization was carried out for the optimum formula of micro-nano mytilus eduli shell powder's preservative, which regards hairtail as object of study and the content of TVB-N as fresh indicator. As a result, it showed when there is 0.65 g micro-nano mytilus eduli shell powder, 1.4g sodium alginate,0.9g CMC, with the content of TVB-N is 6.360mg/ 100 g, the best preservation effect of hairtail appeared in 4d hairtail under 4?.3.The hairtail was divided into preservation group(micro-nano mytilus eduli shell powder's preservative), film group(Alginate preservation agent), and control group(untreated). They were stored under 4 ? for 12 days, and analysis of the physical and chemical indicators and textural properties of the three groups were conducted. The results showed that the sensory evaluation score in the preservation group was significantly higherthan that in the controlled group and agent group(P<0.05) in sensory analysis. In addition to that, consistent results were showed on hairtail odor, p H value and total volatile basic nitrogen. However, the differences of the changes of colors of hairtail and texture of hairtail's meat was not obvious(P>0.05). Therefore, it cannot be regarded as the indictor of evaluating hairtail freshness.4.According to the comparison of different treatment for the three groups hairtail's myofibrillar protein, fish protein total sulfhydryl content and fat oxidational rate, different treated hairtail myofibrillar protein, total sulfhydryl protein content tested showed clearly upward trend with the extension of the preservation time, when good inhibition of myofibril protein degradation appeared with insignificant difference(P>0.05). Comparing the results from the three groups, fat oxidational rate all obviously increased in the three groups, and preservation group can more effectively suppress the speed of fat oxidation.When analyzing biogenic amines and microbial indicators hairtail at different storage time,the content of overall microorganisms, Enterobacteriaceae, staphylococcus aureus, lactic acid bacteria, Microbes and putrescine, cadaverine, cadaverine, tyramine, histamine and tryptamine in the conservation group was found obviously lower than that in the other two groups(P<0.05). Finally, conclusion was made according to the storage of eight days of the three groups of hairtails' SPME-GC-MS analysis. It was found that there was a significant decrease of inherent flavoring substances and a significant increase in biogenic amines in the controlled group compared to the preservation group and film group.In summary, this paper studied a new kind of aquatic preservative based on the micro and nano shell powder and explored its preservation of hairtail. The results was found that the micro-nano mytilus eduli shell powder's preservative had a certain effort in preservation of hairtail, mainly through the inhibition of lipid oxidation rate of hairtail,while fish protein and corruption deterioration did not work. There was also a significant effect on inhibitory effect of microorganisms and biogenic amine and the generation of amines compounds.