| Biodegradable as the way of the new transformation of coal biodegradation by more and more attention in recent years, compared with the degradation of the traditional methods, it is environmental protection, low cost, thus attracting more and more researchers to explore and made some important achievements, but the degradation effect is low, bacteria growth environment is restricted, inhibition of substrate degradation and other issues also followed, these problems always restricts the industrialization development of coal biodegradation; Surrounding coal biodegradation in order to solve this problem, the status quo, first studied the several kinds of methods, improve the effect of biodegradable secondly study the degradation enzyme concentration and enzyme activity in the process of change, the research direction is pointed out that the biodegradation of coal, coal biodegradation and industrialization development specific advice is given.With the rapid development of economy, the growing demand for fossil fuels, mining has many years of low-sulfur coal reserves are dwindling, the use of high sulphur coal become inevitable, and followed by the problem of pollution become the urgent problem, combined forms of sulfur in coal including organic sulfur and inorganic sulfur. Organic sulfur mainly refers to the uniform distribution of organic matter in coal structure, the inorganic sulfur refers to sulfide sulfur (mainly pyrite sulfur FeS2) and sulfate sulfur. Inorganic sulfur out method has been maturing, and organic sulfur out still have little effect, microbial degradation of coal to provide experimental basis for biological desulfurization, the coal Wisconsin model illustrates the basic structure of organic sulfur in coal organic matter, the organic sulfur mainly sulfide, mercaptan and disulfide, etc. So this article tries to study microbial degradation of sulfur-containing model compounds are indirectly to explore microbial degradation of organic sulfur in coal.Paper mainly includes two aspects:one is to study the yellow spore hairs flat leather red pseudomonas bacteria and the ball in different in mutagenesis were enzyme activity and the concentration change. The second is to explore the two kinds of bacteria in the different model of sulfur-containing compounds, the degradation efficiency of degradation products and degradation mechanism. Specific research methods and research results as follows:1. Different ultraviolet mutagenesis time processing conditions, the yellow spore fungus were purified detected three hairs flat leather degradation enzyme content changes as follows:1) LiP concentration induced increases with time, present the first increases then decreases, LiP between 40s-160s generation is bigger, the maximum concentration of 0.0715 mg/ml, continue to increase induced time, concentration of decline.2) concentration of MnP start without too big change, but with the increase of time appear sharply rising,120 s largest enzyme concentration, 0.071 mg/ml.3) Lac concentration as a whole is on the decline, induced bacteria concentrations are lower than the control group. Activity changes as follows:the activity of LiP first induced increasing enzyme activity increase with time, induced time of 80 s largest enzyme activity, and then as time increase the enzyme activity had a downward trend, the activity of MnP mutation increases with time showed a trend of decrease after the first increases, the 120 s when the activity is the largest, but on the whole, enzyme activity change is not particularly significant. Laccase activity increased with the increase of mutagenesis time present the slightly upward trend, in the 120 s when the activity of the largest,250 s is higher than the control group.2. Under the condition of different microwave mutagenesis, after purification, detection produce degradation enzyme activity changes as:degradation enzyme activity changed slightly, the LiP activity change is bigger, the overall showed a trend of decrease after the first increase, and enzyme activity were greater than the control group. MnP activity under LiP, and little change as a whole. Lac basic no change, the activity of the active low. By Inner Mongolia lignite degradation experiments, the degradation rate than the control group increased a little, this that coincide with enzyme activity, further illustrate the enzyme activity and has a direct relationship between coal and degradation rate. Infrared spectrum analysis, the absorption peak of C-O functional groups, that have taken place in a certain degree of oxidation of coal samples,,1450 cm,1526 cm-1-1 of the absorption peaks were aromatic benzene ring frame vibration, aromatic skeleton, aromatic substances from lignite were significantly degraded. Scanning electron microscopy (sem) images the surface uneven, a honeycomb.3. The yellow bacteria spore hairs flat leather of DBT degradation experiment study, yellow as degradation bacteria spore hairs flat leather bacteria and red ball bacteria also has the ability of DBT degradation model of sulfur compounds. Time after treatment in different ultraviolet mutagenesis induced degradation bacteria can also be DBT, and increase with the increasing trend from mutagenesis time, decrease after 80 s the best effect, achieve the degradation rate of 31.5%. Mutagenesis time higher than that of the 120 s, the degradation rate is less than the control group.4. The red ball pseudomonas of DBT exploring experiment, two different desulfurization system is studied, and the appropriate increase content of DBT concentration can improve the degradation, is conducive to make full use of the degradation of system degradation.2) under the condition of different microwave mutagenesis and uv mutagenesis, ultraviolet mutagenic effect is better, and microwave mutagenesis basic little degradation rate fluctuations, the red ball bacteria are more sensitive for ultraviolet mutagenesis. DBT as inducer of domestication experiment design, domesticated microbes degradation rate were greater than the natural strains, played a significant role in DBT as the inducer.5. The red ball pseudomonas DBT degradation of response surface experiment, in the many factors that influence efficiency of DBT degradation, screen out the inoculation quantity, pH and DBT three significant factors, the optimal level combination of 6.5,1.5 ml, tendency for 1.6 L. A, C, A2, B2, C2 effect significantly; B, AC, AB they significantly, the influence of the interaction item BC effect was not significant.6. The red ball bacteria removal of organic sulfur in DBTO2 experiment, through the Gibbs detection, determine product is 2-HBP, further verify the red ball bacteria degrade DBT is 4 s, by contains DBTO2 and intermediate. Red ball bacteria of DBT02 desulfurization ability and DBT is the same. By the mixed bacteria degradation experiment research, can be found with the yellow spore fungus spore suspension liquid hairs flat leather product increases, the color of the solution gradually deep, this is the same as the spectrophotometer detection results, shows that with the increase of yellow bacteria volume, red ball bacteria to DBTO2 desulfurization efficiency increased. Ultraviolet mutagenesis condition, the red ball bacteria mutagenesis of bacteria on the desulfurization effect of DBTO2 showed a trend of decrease after the first increase, in mutagenesis for 60 s with maximum efficiency, desulfurization effect between 60s-160s. Microwave mutagenesis condition, the degradation rate of a wave of change, but the overall degradation rate increased. |
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