The Design And Application Of Fluorescent Probe For Human Serum Albumin Based On Hydroxychalcone

Fluorescent probes with unparalleled advantages such as highly sensitivity and selectivity has been important tools for the dectection of various analytes, especially for the optical imaging of biomacrolecule such as protein and DNA in vitro and vivo.Human serum albumin (HSA) is the most abundant transport protein in the circulatory system which plays a key role in various biological systems, such as maintaining osmotic pressure, transporting of endogenous and exogenous substances. The practical methods for sensitive and accurate detection of HSA in complex biological samples are highly desirable.A fluorescent probe HCAB based on twisted intramolecular charge transfer (TICT) mechansim has been designed and synthesized by introducing benzoyl moiety to 4-Dimethylamino-2'-hydroxychalcone. Upon interaction with Human Serum Albumin (HSA), the probe HCAB showed 88-fold fluorescence enhancement due to the inhibition of TICT. The probe shows excellent selectivity towards HSA among 18 different enzymes with a wider linear range 0-300 mg/L and a low detection limit of 1.05 mg/L. Molecular docking and the displacement assay were conducted to display the interaction site between HCAB and HSA was site I, which was located in subdomain IIA. The practical application showed that probe HCAB can be used to detect and quantify trace HSA in blood plasma.A fluorescent probe A based on acenaphthene-heterocyclic derivatives has been synthesized by the nucleophilic addition between carbonyl and amino group with the hope of the increase of conjugated system which would lead to red shift. And the route of synthesis of probe A was optimized.