| Silver carp is one of the four major carp in China, is widely used in fish products. Studies shown that fish oil(FO) of silver carp is rich in polyunsaturated fatty acids(PUFAs), It can serve as a good source of PUFAs of product development. As a novel type of encapsulation technology, liposome has the following advantages: protecting effective component activity, enhancing the bioavailability of active components, masking the unpleasant odor, prolonging the shelf life of active components, and directional targeting release. The use of liposome to encapsulated fish oil can effectively make up the defects of fish oil, such as easily oxidation and bad odor.This research used fish oil of silver carp as raw material, which was treated by alkaline catalysis method to prepared fish oil ethyl ester. The ethyl ester fish oil was enriched PUFAs by urea addution method. The fish oil nanoliposomes was prepared using ?-Sitosterol instead of cholesterol and richment of fish oil as core by film dispersion method combined with Dynamic high pressure microfluidization(DHPM) technology. Finally, comparative ?-Sitosterol-fish oil nanoliposomes and cholesterol-fish oil nanoliposomes in the entrapment efficiency, particle size and storage stability of different.(1) Fish oil from Silve Carp was used as the raw material to study unsaturated acid esterification catalyzed by sodium hydroxide. By the single factor experiments and the orthogonal experiments, the best conditions for fish oil ethyl ester were obtained as following. The addition amount of sodium hydroxide was 0.2%(w/w) of fish oil weight. The molar ratio of methanol to oil was 8:1. The reaction temperature was 75 ?, and the reaction time was 1.5 h. The yield of ethyl ester was 97.47 %.(2) Using fish oil from silver carp(ethyl ester) as raw materials, PUFAs was enriched by urea addution method. The optimal process parameters were determined through single factor and orthogonal experiments. The optimum parameters were as follows: the volume-to-mass ratio of 95% ethanol-urea was 6:1, the mass ratio of fish oil-to-urea was 0.3:1, inclusion time was 3 h, inclusion temperature was 10 ?.Under the optimal conditions, content of EPA in fish oil with initial 14.47% increase to 43.68%; DHA by original 8.62% increase to 29.99%; fish oil recovery rate 22.93%; the purity of EPA and DHA reached 73.67%(3) Using lecithin??-Sitosterol?Tween-80 as wall material, enrichment of fish oil as core material, the preparation of fish oil nanoliposomes by thin film dispersion-DHPM was adopted. The optimal process parameters were determined through single factor and orthogonal experiments, as follow: phospholipid concentration was 25 mg / mL, the mass ratio of lecithin to sterols was 7:1, the mass ratio of lecithin-to-fish oil was 6:1, Tween-80 accounted for 12.5% of the mass of lecithin, pH of PBS 7.5, pressure of DHPM was 120 MPa for 3 times. Under the optimum preparation conditions, the encapsulation efficiency of the nano liposomes was 94.05 %, and the particle size was 88.9 nm.(4) The encapsulation efficiency, particle size, pH of ?-Sitosterol nanoliposome and cholesterol nanoliposome in 4 and 25 for 35 days were investigated.? ? The results showed that the storage stability of ?-Sitosterol nanoliposomes and cholesterol nanoliposomes was better at 4 ?, and ?-Sitosterol could be used instead of cholesterol in the preparation of fish oil nanoliposomes. |
PDF Full Text Request