Rapid Detection Of Salmonella By Carbohydrate Microarray

The last decade has seen a vast number of human and animals around the world sufferd from diseases caused by salmonella, which is considered as the main food-borne pathogens of both human and animals. These diseases have resulted in great financial loss as well as public health hazards. So it is important to develop a rapid method with higher sensitivity, specificity and excellent repetition for the detection of the salmonella.Numerous studies showed carbohydrates play a critical role in many biological processes. Therefore, studying the interactions between carbohydrates and other biomacromolecules can contribute to biological and medical investigations. However, it's difficult to study carbohydrates due to their complexity of structures and functions. Carbohydrate microarray is one of the valuable tools to study the interactions of carbohydrate and other biomacromolecules. It has been shown that this method has many advantages such as low amount of samples required, high specificity and high throughput screening. Nowadays, the study of carbohydrate microarray is the first stage in the world, few studies in the detection of bacteria. Therefore, the establishment of carbohydrate microarray technology for the detection of salmonella is meaningful.We utilized the interaction between FimH-mannose and prepared carbohydrate microarrays by chemical modification and covalent immobilization. In the process of constructing carbohydrate microarray selected epoxidized and amicinated array to determine the optimal conditions. Herein, we investigated the application of carbohydrate microarrays in salmonella detection. Salmonella ATCC31685 was mainly used for the tests. First, different kinds of carbohydrate microarrays were prepared to optimize the detection conditions of this method. On this basis, the detection limit value was determined by using different concentration of carbohydrates or bacteria. Then we studied the application of carbohydrate microarray in screening salmonella strains and FimH antagonist, and investigated the effect of carbohydrate microarray on bacteria strains. The results showed that salmonella strains can conjugate with the carbohydrate microarray functionalized with D-mannose. And the carbohydrate microarray prepared by Man-1 and NHS can conjugate with the strains best. The two kinds of salmonella strains (ATCC31685 and ATCC9184) have different abilities to bind with carbohydrate microarray. Therefore, the carbohydrate microarray can be a fast and effective mean to detect salmonella. The fluorescence intensity can be still distiguinshed from the background when the concentration of carbohydrate is 313 ?mol/L or the concentration of the bacteria is 106 cells/mL. The nondestructive nature of carbohydrate microarray allows the detected salmonella to be harvested and tested for antibacterial susceptibility. In addition, the results showed that the carbohydrate microarray can be used for screening FimH protein antagonists.