The Research Of Encapsulation Property Of The Lipid Carriers For The Water-soluble/Oil-soluble Ingredients

Liposomes are widely used in medicine and cosmetics because of its unique bilayer structure. Now the liposomes has some shortcomings such as large particle size, low coating rate and a low concentration which is difficult for large-scale production in field of cosmetic.lt has important practical value to study a liposome system which can be used in cosmetic safely and effectively.This study used 1,2-propylene glycol as a lecithin solvent which was a humectant in cosmetics, and prepared blank liposomes. Based on this study, the effect of preparation, formulation and composition additives on the particle size and stability of the liposomes were studied and the preparation process and composition of liposome were optimized, the final results were as follows:room temperature; ultrasonic time was 12min and ultrasonic power was 225w, the addition of Tween80 was 0.33%, NaCl was added in an amount of 0.05%, the amount of water-soluble oil PEG-7 glyceryl coconut oil was 1%, and under these condition, a high concentration (10%), small particle size (66nm) and stable liposome system can be prepared. On the basis of empty liposomes, an oil-soluble active ingredient glycyrrhetinic acid and water-soluble active ingredient D-panthenol, were encapsulated into liposomes, while the indexes of particle size, encapsulation efficiency and the stability were used to optimize the preparation process and formula composition, respectively, the application performance of both liposomes were studied as well. Best preparation and formulation of glycyrrhetinic acid liposomes were as follows:ultrasonic time was 2min; ultrasonic power is 225w; Tween80 amount was of 0.33%; NaCl was added in an amount of 0.05%; the amount of PEG-7 glyceryl coconut was 4%. Particle size of prepared glycyrrhetinic acid liposomes was (117± 4.64) run, encapsulation efficiency was (91.0 ± 1.21)%, the particle size of produced liposomes was uniform, spherical vesicles. FTER. results showed that glycyrrhetinic acid was coated into liposomes; the in vitro release experiments showed that glycyrrhetinic acid liposomes had a significant slow-release effect, transdermal experiments showed GA in GA liposomes possessed a high content in the dermis and could act effectively in the dermis; stability results showed that glycyrrhetinic acid liposomes prepared at storage condition of 4? had rarely changed in 30days and showed good stability. The best preparation and formulation composition of D-panthenol liposome were as follows:the ultrasonic time was 2min; ultrasonic power 225w, the amount of Tween80 was 0.33%; NaCl was added in an amount 0.05%. The in vitro release experiments showed that D-panthenol uposomes had significant sustained property. In vitro transdermal experiments showed that D-panthenol liposome had higher retention in the skin, and could effectively act on the dermis.