Effects Of SA And 1-MCP Treatment On Ripening And Softening Of Apricot Fruit And Cloning, Expression Analysis Of PG And PME Genes

Xinjiang apricot fruit(Saimaiti) were taken as the test materials in this paper. The effect of apricot fruit on the cold storage quality, quality shelf life at room temperature, cell wall metabolism-related enzyme activity and the key genes related to softening were also cloned and the expression level were researched with 1-MCP and SA. PG and PME genes function and regulation of physiological mechanisms were explored preliminary in apricot fruit softening. Further clarify the mechanism of ripening and softening apricot fruit is important, this provide a theoretical basis for control after ripening and softening apricot fruit. The main results were as follows:(1) During the cold storage, 1-MCP and SA can control apricot fruit quality decreases effectively. The results showed the soluble solids, weight-loss ration, cell membrane permeability, respiration rate and ethylene production were inhibited than those of the control group, the fruit firmness, titratable acid content and chlorophyll content were also delayed to decline by 1-MCP and SA treatments, and the effects of1-MCP were better than SA treatment.(2) During the cold storage, the PG, PME, ?-glucosidase activity were inhibited effectively by 1-MCP and SA treatments and cellulase activity were delayed by 1-MCP and SA treatments compared with the control group, the protopectin content were delayed to decline by 1-MCP and SA treatments, the soluble pectin content were also delayed to rise by 1-MCP and SA treatments. 1-MCP and SA treatments have a significant inhibition of softening and the mature aging in apricot fruit.(3)The results showed the weight-loss ration, cell membrane permeability, respiration rate and ethylene production were also delayed to decline by 1-MCP and SA treatments during shelf-life, while the decrease of the firmness, titratable acidity, chlorophyll and commodity rate were also delayed by 1-MCP and SA treatments, but soluble solids content was little changed, and the effects of 1-MCP were more obvious.(4) Pa PG1(Genbank accession number: KR153990) and PaPME1(GenBank accession number:KR153991) full-length gene sequence were cloned from ‘Saimaiti' apricot fruit. The full-length gene sequence of PaPG1 was 1182 bp and the full-length gene sequence of PaPME1 was 1114 bp.(5) By quantitative real-time PCR to detect the expression levels of PaPG1 and PaPME1 genes by1-MCP and SA treatments were less than the control group, this results indicated that PaPG1 and PaPME1 genes expression were inhibited to rise by 1-MCP and SA treatments, and it showed that 1-MCP treatment is better than SA.