Novel Gold Nanorods-Based SERS Probes For Living Cells Imaging And Detection

Surface-enhanced Raman scattering (SERS) has become a mature vibrational spectroscopic technique during the last decades and the number of applications in the chemical, material, and in particular life sciences is rapidly increasing. One of the fundamental goals of biochemistry, biophysics and molecular biology is to understand the complex spatiotemporal interactions of molecules at biological interfaces. To study these interactions, researchers commonly use in vivo cellular imaging and in vitro assay detection. Molecular images of cells, tissues, and organs, and the body are able to monitor various structures or mechanisms related to pathology. There is strong interest in the development of highly sensitive imaging optical technologies in biomedical diagnostics, pathogen detection, gene identification, gene mapping and DNA sequencing. protein receptor, can not be monitored. Raman imaging is an emerging field that has generated a lot of interest both for the label-free Raman methods and for the unique properties of nanoparticles applied as contrast agents and Raman reporters of proteins and nucleic acid targets. This paper explains two GNR-based SERS probes for living cell imaging detection.(1) A Novel SERS Nanoprobe for Ratiometric Imaging of Hydrogen Peroxide in Live Cells and Cancer TissueHere designed a gold nanorod (GNR)-based SERS probe via the assembly of 4-mercaptophenylboronic ester (MPBE) on the GNR's surface for H2O2 imaging in live cells or cancer tissues. boronate ester has been recognized as a specific recognition element for H2O2 based on the chemo-specific boronate-to-phenol switch to respond to H2O2. thus resulting in the decrease or disappear of the strong Raman band at 993 cm-1 attributed to the B-O symmetric stretching while at the same time the Raman band at 1071 cm-1 attributed to C-H in-plane deformation is not changed clearly, then realizing the quantitative detection of H2O2 via the relationship between H2O2 level and the ratiometric peak intensity of 11071/993, realizing ratiometric imaging of H2O2 in living cells or tissues.(2) A Novel GNR/Alkyne-based SERS Nanoprobe for Raman Imaging in Live CellsHere prepared a novel bioprobe by combining alkynes and gold nanorods, as an example of an alkynes-nanoparticle SERS probes. The SERS probe is created by the well-controlled coadsorption of alkyne-contained dyes onto gold GNR surface which are then silicon wrapped to create a very stable colloidal suspension. relatively hydrophobic dye molecule 1,4-Diphenyl-1,3-butadiyne is readily sequestered into the CTAB bilayer when introduced to an aqueous nanorod solution. The nanorod-dye conjugate yields strong SERS signals of alkyne, the alkyne moiety shows a distinct, strong Raman scattering peak in a cellular silent region (1800-2800 cm-1), where most endogenous molecules show no Raman scattering.The nanorod-dye conjugate is then stabilized by wrapping with a layer of silicon. Correlated Raman mapping measurements of SERS probes in living cells and tissues were used to clearly demonstrate that strong and reproducible SERS signals were readily achievable. The silicon-wrapped nanorod-dye conjugates were also found to be highly stable as well as noncytotoxic.