| This laboratory conserves a bacteria strain which can efficiently degrade organic phosphorus pesticide chlorpyrifos.After strain identification,it identified as a new species,named Cupriavidus nantongensis X1.In this paper,the degrading characteristics of the strain to several other organophosphorus pesticide was studied.Fluorescent labeling was carried out by chemical conversion method to the strain and the genetic stability was measured in order to study labelled strain 's migration dynamic and changes of degrading ability to chlorpyrifos in the migration process.Through extracting Cupriavidus nantongensis X1's RNA from the soil,the minimum amount of strains was determined.The results showed that 9 kinds of organic phosphorus pesticide which have been selected can be degrade by the Cupriavidus nantongensis X1 strain.Degradation rate of Parathion,Triazole-phosphorus and Isocarbophos in 24 h reached 100%,Profenofos' s degradation rate reached more than 60%,Degradation rate of Fenthion,Malathion and Cadusafos were about 45%,Methidathion and Dimethoate had a low degradation rate,only around 20%.Fluorescent genetic stability study showed that,exogenous plasmid with luxAB gene has genetic stability.Using luxAB gene marker to assist research is feasible in follow-up study of soil environmental behavior.Picked single colony from the plate in the fluorescent genetic stability study,inoculated it in LB liquid medium which is containing 20 mg·L-1 tetracycline to enrichment culture.Measured the degradation of Chlorpyrifos by 10 times switching strains in 12 h.The results showed that in 10 generations of subculture,marked strain's degradation efficiency has no decline,200mg·L-1 chlorpyrifos degradation rate in 12 h was maintained at about 50%.Successfully extracted total RNA of the strains that has been inoculated into sterile soil.Extracted the required amount of RNA,at least need to inoculate 105 cfu·g-1 bacteria into soil. |
PDF Full Text Request