| In this thsis, a nano composite material was made by hydrothermal method?GO-ZnS?, which is the preparation of grapheme oxide and zinc sulfide quantum dots. In order to obtain the GO-ZnS nanocomposite modified glassy carbon electrode?GCE?, the surface morphology and composition of this material were analyzed by using scanning electron microscopy?SEM?, X ray diffraction?XRD?.And this greatly improved the voltammetry glassy carbon electrode response. A new type of biosensor was prepared to enhance the effect of electrode interfacial electron transfer based on GO-ZnS. And this realized the detection of environmental priority pollutants. The main contents are the following three aspects.The modification effect of graphene oxide?GO? is the best, judging by the comparison of the graphene oxide?GO?, platinum?Pt? nanoparticles, gold nanoparticles and chitosan?Au-CTS?. And then used it as a crosslinking agent, when GO and DNA respectively 5mg/mL, 5mg/m L, adsorption potential and adsorption time of DNA were 0.50 V, 200 s, DNA/GO/GCE composite film sensor by chronoamperometry obtained in pH=7.00 PBS of acrylamide?AA? responsed to the best effect. Using differential pulse voltammetry?DPV? method for the determination of the electrochemical signal, acrylamide?AA? and the size of the logarithmic concentration showed a good linear relationship, the response was in the range of 5*10-81.0*10-3mol/L, the linear equation was I =4.9286+0.4245 lgc, the correlation coefficient was 0.9989.GO-ZnS nano composites were prepared by hydrothermal method, the SEM, XRD display and uniform ZnS quantum dots doped into the GO. To examine the GO, ZnS, GO-ZnS were modified glassy carbon electrode?GCE? for DNA/GO/GCE, DNA/ZnS/GCE, DNA/GO-ZnS/GCE composite film sensor differential pulse voltammetric behavior, determine that the modification effect of GO-ZnS nano composite material was the best. When the concentration of GO-ZnS and DNAwas respectively 6mg/m L, 4mg/mL,scanning speed is 50mV/s, the DNA/GO-ZnS/GCE composite film sensor in acetate buffer solution pH=4.60 in the range of Ni compounds responsed to 5*10-71.0*10-3mol/L, the linear equation was ? I =4.8176+0.4272 lgc, the correlation coefficient was 0.9949.By using electrochemical method, DNA/GO-ZnS/GCE composite film sensor in acetate buffer solution pH=4.60 in the range of AA-Ni composite response was 1.0*10-7 1.0*10-3mol/L, the linear equation was ? I =4.5333+0.3934 lgc, the correlation coefficient was 0.9931. The determination of guanine AA-Ni composite system of the sensor?G? on the oxidation peak current of the difference acrylamide?AA? was slightly lower than the single system and Ni compound with a single system of guanine?G? on the oxidation peak current of the difference, and the composite system in the response sensitivity of acetate buffer solution pH= 4.60 was significantly higher than that in pH=7.00 phosphate buffer solution?PBS? in the response sensitivity. |
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