Anodic Electrogenerated Chemiluminescence Biosensor Research Based On Self-Assembled Peptide Nanomaterials

ECL has been widely used by integrating advantages including highly sensitive, specificity, rapid testing speed. In recent years, the main study direction of ECL contains the preparation and development of optical materials, the expansion of effective coreactant, the way of immobilizing the optical materials, ECL mechanism and utilization in imaging technique. Various new ECL emitters such as silicon nanocrystals, carbon QDs and Au nanoclusters as well as organic luminophores, have been developed successively. However, the ECL emission efficiency of these inorganic nanoemitters is lower. Although organic ECL emitters have much higher emission efficiency than inorganic emitters, the stable ECL emission in an aqueous system was obtained with difficulty based on these organic ECL emitters so far. This largely limits the application of organic ECL luminophores in biological analysis. Therefore, the development of new, highly efficient, nontoxic and tunable ECL emitters is still highly desirable.Diphenylalanine dipeptide (FF) is the simplest peptide block, which can self-assemble into nanomaterials with diverse morphology. The self-assembled materials contain unique optical properties attribute to the quantum confinement effect which conduce to the application in the ECL sensor. Therefore, this thesis based on the FF self-assembled nanotubes, nanowire arrays and the FF's derivative self-assembled hydrogels combined graphene quantum dots, exploring the anodic ECL performance of them and applying in the analyzing detection. This work is demonstrated in detail:1. Anodic ECL performance of the self-assembled peptide nanotube(PNT) modified electrode in an aqueous system was observed using tri-n-propylamine(TPrA) as the coreactant. The mechanism and the luminescence property was explored. The potential application of PNT in analytical chemistry was also demonstrated using Cu2+ as an example.2. Exploring the anodic ECL performance of the FF's derivative (Fmoc-FF) self-assembled hydrogel(DP HG) with TPrA as the coreactant. Then the DP HG were used for the immobilization of GQD, achieving GQD/DP HG composite material. The composite material showed better ECL property owing to the synergistic effect of the two luminescent materials. Choosing A?(1-40) as the model analyte, we achieved the ECL biosensor with advantages of highly sensitive, wide linear range and excellent anti-jamming ability.3. Prepared the FF nanowires arrays(PNWs) and fixed it on the glassy carbon electrode (GCE), then we explored the anodic ECL property of the PNWs choosing TPrA as the coreactant. PNWs, the new luminous material, realized the sensitive detection of DA and broadened the application of FF self-assembled materials with different morphology structure in the field of ECL biosensor.