The Design And Application Of Signal Amplification By Exploiting The Conformational Change Of DNA

With the development of life science and analysis technology,trace detection is particularly important in the biological analysis and research,so it is very eager to improve the sensitivity.The structure of DNA is foldability,diversity and identifiability,thus it is important to design and apply signal amplification in biosensor based on the change of DNA structure.The main content is to combine the various amplification method with DNA based biosensor,the amplification method include enzyme assisted cycle amplification,hybridization chain reaction and so on,and then we can develop the high selectivity,high sensitivity sensor analysis method by the colorimetric and fluorescent.And then we can introduce the following several aspects:（1）Introduction the concept of biosensor and optical（fluorescent,colorimetric）DNA biosensor,the design and amplification of various signal amplification method.（2）Design exo-Ⅲ assisted multi-cycle-amplification,combine nucleic-acid-sensitized terbium’ fluorescent system and the Fe₃O₄ magnetic nanoparticles assisted background decreases,we construct a high sensitivity DNA biosensor for the detection of DNA.（3）（a）Based on the electrostatic interaction between coralyne and heparin and the conformational change of DNA,we design a novel biosensor for the detection of heparin.（b）By the fluorescent quenching effect of MnO₂ nanosheet and the signal amplification of HCR,we presented a ultrasensitivity optical biosensor for the detection of heparin.（4）Based on the effect of ssDNA or dsDNA toward the nanozyme activity of MnO₂ sheet,we uccessfully construct a colormetric system for the detection of various biomolecular.