Charicterization Of “Microbial-metal Oxides” Interface Interaction And Its Application

Microbes play important roles in the environmental biotechnology. There are many forms of interface reaction in the environment microbiology technology that affect the attachment of microorganisms on the surface of the media. Studying the interface reaction of microbes and functional materials is useful to solve a series of environmental problems, such as membrane fouling, biological corrosion, and it also helpful to develop new microbial selective enrichment and separation technologies, which play a very important role in microbe detection and environmental risk early warning.In this study, nano magnetic bead based separation technology was developed, to effectively separate microorganisms on the glass fiber filter membrane based on the DLVO theory. Under acid condition, the magnetic nanoparticles adsorption microorganisms on the the glass fiber filter membrane has maximum yield and enables the adsorption of microorganisms without discrimination.Combined with the classical filtration theory and chemical properties of siderophore on the surface of the metal oxide, studying the microbial adsorption behavior characteristic mediated by the siderophore. Construction of microbial adsorption characterization based on filtration experiment system. The strain EPS- with a dsficiency in EPS production and the strain EPS+ show the different filter form in glass beads filter. EPS- is compatible with the log-linear decrease pattern anticipated from the classical filtration theory, but strain EPS+ effected by EPS that show some discrepancies from the classical filtration model, which had a inflection point located at 15 cm. In the experiment of deferoxamine B modified filter material, the wild-type strain BW25113 show a non-classical filtration model, which has a non-monotonic change of constant deposition rate coefficient. The retained cell concentration firstly increased and then decreased, with the maximum retained concentration occurring at 15 cm. As control,in the non-functionalized filter material the retained cell concentration also firstly increased and then decreased, with the maximum retained concentration occurring at 10 cm. Comparing with the control group, retention BW25113 bacteria in the experimental group improved significantly by 50%.Based on the affinity of siderophore on microbial, development of siderophore modified materials, to explore the feasibility of selective adsorption of specific microorganisms by the modified materials. Preparation of siderophore modified magnetic nano-material, in mix system consist of strain fluE++ with overproducting of receptor proteins of DFOB and strain fluE- with a dsficiency in production of receptor proteins of DFOB by the concentration ratio 1:3. Modified magnetic beads to preferential adsorption fluE++ strains, adsorption of fluE++ and fluE- with a ratio of 4:1, much higher than the Fe₃O₄ absorption amount of fluE++ Using the siderophore modified magnetic nano-material treat the water of Shenzhen Bay, In acidic and neutral conditions, the amount of adsorpted Actinomycetes which is the most easy to combine with DFOB in water of Shenzhen Bay significantly higher than alkaline condition and adsorpted by Fe₃O₄...