Study Of Capillary Electrophoresis And Thin-layer Chromatography Fingerprint On Herbal Tea Beverages

Herbal tea, a convenient Chinese beverage, is produced by Traditional Chinese Medicine(TCM) with the function of thirst slaking and heat relieving. Since the complex TCM constituent and ratio, a set of standard quality control must be set up by using modern analytical technology. Fingerprint, as a very know modern analytical technic, was effectively used in TCM quality control and preparation process, and also it will become the most popular method in this area. Capillary electrophoresis(CE), thin layer chromatography(TLC), high performance liquid chromatography(HPLC) and gas chromatography(GC) are distinctive methods for constructing fingerprint. The herbal tea beverages fingerprinting technique is a practical, scientific and advanced quality assurance and inspection system based on the research work of TCM fingerprint. In this report, the characteristics of fingerprint and its application in TMC constituent analysis were discussed. We also investigated CE, HTLC and METLC segregation analysis methods for herbal tea beverages. The specific contents are as follows:(1) Effects of several important factors such as the type and concentration of running buffer and additive, operation voltage, injection time and detection wavelength were investigated. The herbal tea beverages extractive can be detected in the condition of 18 k V operation voltage, 5 s injection time and 214 nm detected wavelength, 60 mmol/L sodium borate 12.5%(v/v) methanol(p H=8.2). The herbal tea beverages fingerprint of CE is established basing on the result analysis of 10 batches herbal tea beverages isolation.(2) MTLC separation experiment was carried out by using various micellar solution as developer, and the micelle developer was optimized by improved simplex method. The best developer was 0.067 mol/L SDS+21%(v/v) n-propanol +18%(v/v) acetic acid, and the herbal tea beverages' ingredients was separated under this condition. Based on the 10 batches of herbal tea beverages isolated analysis, the herbal tea beverages fingerprint of MTLC is established.(3) The oil phase composition was added in the micelles developer, and then the composition of METLC developer was optimized. The composition of METLC developer was 0.1M SDS+18%(v/v) butanol+20%(v/v) acetic acid+2.4% n-heptane. The separation system of METLC was established for the herbal tea beverages and the herbal tea beverages fingerprint of METLC was built up.(4) The optimal composition of microemulsion developer was obtained by changing the water proportion and modifiers type. Another optimized composition of METLC developer was 70% water content of microemulsion(SDS + n-butyl alcohol + n-heptane mass ratio 27:63:10) + acetic acid(9:1). Another separation system and fingerprint analysis of METLC was established for the herbal tea beverages.