The Influence Of Different Pretreatment On The Enzymatic Hydrolysis Of Sugarcane Bagasse

The wide use of the fossil fuels largely promoted the development of the whole world, however, aroused serious environmental problems. Hence, more attention had been paid to the renewable bioethanol. Bioethanol, which has high octane value, can be blended with gasline as transportation fuel. In addition, it also has many advantages for the conversion of biomass to bioethanol, such as decreasing lignocellulosic waste, development of agricultural economy, decreasing CO2 emossion. Sugarcane bagasse(SCB) is one of the major byproducts of the sugar industry. It has been an important renewable biomass resource due to its wide variety of sources and abundant content of homocellulose. Because of the sealed structure of lignocellulose biomass composed of cellulose, hemicellulose, and lignin, the enzymatic hydrolysis efficiency of biomass is low, pretreatment is required to disrupt the intact structure, and improve the efficiency of enzymatic hydrolysis and bioethanol yield.In this study, Coriolopsis gallica pretreatment of SCB were proposed and compared with other pretreatments. Based on the results of composition analysis,SEM analysis, FTIR analysis, as well as the enzymatic digestibility, the influence of different pretreatments on the efficiency of enzymatic hydrolysis were examined.The investigation on Coriolopsis gallica pretreatment showed that pretreatment time, seed dosage, and water significantly affected the emzymolysis efficiency of the pretreated SCB. Three factors and three levels of orthogonal experiment indicated that the optimal conditions for Coriolopsis gallica pretreatment were: solid-seed ratio of1:0.5, time of 25 d, and the solid-water ratio of 1:2.5. Compared with the raw SCB,lignin decreased by 30.07% after pretreatment, the sugar yield during enzymatichydrolysis reached 29.40%. At the same time, sodium hydroxide(Na OH)pretreatment, dilute sulfuric acid(H2SO4) pretreatment and liquid hot water(LHW)pretreatment were also implemented to improve the enzymatic digestibility of SCB.The sugar yields after enzymatic hydrolysis reached 81%, 37%, and 63%, respectively,suggesting that Na OH pretreatment significantly affected the emzymolysis efficiency of the pretreated SCB.