The Intervention Of Pleurotus Eryngii Polypeptides On Bone Of Lead Exposed Rats

[Objective] To investigate the effects of Pleurotus eryngii polypeptide on rats bone metabolism changes at molecular and cellular levels caused by lead exposure. To provide theoretical basis of meals is helpful to prevent Lead damage.[Methods] The quality finger map analysis of Pleurotus eryngii polypeptide. In the test,27 healthy 21 days male rats were randomly divided into three groups, and named as control group (normal diet, distilled water), lead exposed group (normal diet,0.2%(CH3COO)2Pb-water), Testgroup (2%Pleurotus eryngii polypeptide in diet,0.2%(CH3COO)2Pb-water).After 60 days, the blood and bone of the rats were collected and the weights were weighed. The osteocalcin, calcitonin and parathormone levels and alkaline phosphatase activity in serum and bone were also detected.The contents of Pb, Ca, Zn, Mg, Fe,Cu and Mn in bone were determined by ICP. The bone histopathological changes were observed by HE stain. In addition, the expression levels of TRPV5, TRPV6, NCX1 and PMCAlb were researched by real-time RT-PCR.[results] (1)The molecular weight of Pleurotus eryngii polypeptide is fluctuated from 500 to 3000 Dalton.(2) Compared with control group, the body weight in lead exposed group was significantly decreased (P<0.01) from the 18th day; Compared with lead exposed group, the body weight were extractly significant increased (P<0.01) from the 24th day in test group.Compared with control group, the body weight growth in lead exposed group was significantly decreased (P<0.01) from the 6th day; Compared with lead exposed group, the body weight were extractly significant increased (P<0.01) from the 12th day in test group.Compared with control group, BGP, CT contents and AKP activity in serum and bone of lead exposed group were extractly significant decreased (P<0.05 or P<0.01), PHT contents was significantly increased (P<0.01); Compared with lead exposed group, BGP, CT contents and AKP activity in test groups were increased, PHT contents were decreased, the AKP activity was significant level(P<0.05).(3) Compared with control group, Pb in bone of lead exposed group was much significantly increased (P<0.01), Ca, Zn, Cu, Fe contents were significantly decreased(P<0.05 or P<0.01), Mg and Mn contents were decreased, but there were no significant difference; Compared with lead exposed group, Pb contents in test group were significantly decreased(P<0.05), Ca, Cu, Zn, Fe contents in test group were increased, but not significantly.(4)Compared with normal control group, in lead exposed group, the bone marrow cavity fuzzyboundary; bone trabecular sparse,partial disappear,atrophy and not smooth. Compared with lead exposed group, the bone marrow cavity shrink; the bone trabecular number increase and got smooth, partial connect into a network.(5) Compared with control group, the mRNA expression level of TRPV5 and TRPV6 in lead exposed group were significantly decreased (P<0.01), the mRNA expression level of NCX1 and PMCA1b in lead exposed group were significantly increased or extractly significant increased (P<0.05,P<0.01); Compared with lead exposed group, the mRNA expression level of TRPV6 in testgroup were significant increased (P<0.05),the mRNA expression level of TRPV5 in test group were significantly increased (P<0.05), the mRNA expression level of NCX1 and PMCA1b in test group was decreased,but not significantly decreased.[Conclusion] In conclusion, the results of the present study demonstrated that Pleurotus eryngii polypeptide could control bone metabolism related biochemical indicators in rats and alleviate the plumbism. Meanwhile, it could regulate the expression of TRPV5, TRPV6, NCX1 and PMCA1b genes. These findings could signify that Pleurotus eryngii polypeptide could relieve the bone injury caused by plumbum.