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Isolation, Identification Of H2S-oxidizing Bacteria And Its Application In Bio-trickling Filter Purifying The Air Polluted By H2S

Posted on:2017-02-23Degree:MasterType:Thesis
Country:ChinaCandidate:Y Z GuoFull Text:PDF
GTID:2311330512464132Subject:Biological engineering
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With the rising of odor pollution incidents and folks' awareness of the harm of it, a high efficient, wide-range, environmental-friendly treatment technology to deal with these challenges is in need. Utilizing the microorganism's metabolism activity by biological filtration tower to absorb and decompose the odor substance is of mild reaction condition, which can treat different kinds of gases and produce no pollution. So the biotrickling filter is worthy of further study?This paper conducted strains screening from the most common odor of hydrogen sulfide and isolated a strain of H2S oxidation bacteria T1 from a sewage treatment plant activated mud aeration pool, through physiological and biochemical tests and 16S rDNA test, we determined the strain as Thiobacillus thioparus. Getting the he optimum growth conditions of strain T1 by orthogonal experiment, for the growth of temperature is 30?, pH 6.5, the medium added Na2S2O3 10g/L.In the process of equipment design, the biological filter tower composed of gas generator, drop filter tower and spray liquid circulation system, was built. The operation parameters of inoculated strain T1 in biotrickling filter tower was ensured by experiments:?1? optimum nitrogen source NH4NO3 ?2? spraying quantity 0.76-1.02L·h-1·L-1?3? H2S concentration under 1500 mg/m3, purify rate over 90%, gas retention time not less than 60s?4? biological trickling filter purification rate was highest with PVA when the volume is the same.In this paper, rule of the biofilm extracellular polymers in microbial biomass, polysaccharide and protein changes with the rising of the H2S concentration by experiment. Simulating the three situations process temperature drop, pollution load swells and equipment may occur in actual production, and studying the buffering capacities with strains T1 inoculated in biological trickling filter tower. To provide basis for further research.
Keywords/Search Tags:Hydrogen sulfide, Biological filtration tower, Thiobacillus thioparus, Extracellular polymeric substances, Buffering capacity
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