| In order to obtain industrial wastewater,This study composite the Bacillus of cellulose production ability and the fungus pellet of lignin degradation ability,forming immobilization mycelial pellet,and study for the performance of the treatment about all kinds of wastewater.First of all,the screening,identification and application of lignin degrading bacteria were studied.The sludge samples were separated and identified by 18 srDNA as Aspergillus fumigatus(Aspergillus fumigatus),named G-13.The best pelletization conditions were obtained by investigating the effects of different nitrogen sources,carbon sources,pH value of medium,inoculum amount,rotational speed and temperature on G-13 formation.The result was Sucrose as carbon source,the concentration was 18 g/L,ammonium tartrate as nitrogen source,the concentration was g/L,the culture medium was pH value of 5,the inoculum was about 5 mL,the rotational speed was 160 r/min and the temperature was about 30 °C.Under the optimum conditions of mycelial culture for 72 h,the maximum diameter of the mycelium pellet was about 3.4 mm,its surface was smooth and had good elasticity which could be used as biological immobilization carrier.In our research,enzyme production ability of immobilization mycelial pellet was used as index,the immobilization mycelial pellet was immobilized with lignin-degrading fungus G-13 and cellulose-degrading bacteria X10-1-2.The influence of five factors(addition time,inoculation amount of cellulose-degrading bacteria,pH,temperature and rotational speed)were study for mycelium pellet.The results indicated that X10-1-2 and G-13 were added simultaneously and the inoculation amount was 20%,pH was 5,culture temperature was 28?,rotational speed was 160 r/min,the manganese(MnP),laccase(Lac),lignin peroxidase(Lip),cellulose,X10-1-2 and G-13 have the complementary advantage on the immobilization process,develope the synergy effects and promote the production of ligninase.the immobilization mycelial pellet was white,smooth surface,uniform size and good elasticity,it can satisfy the need of practical.The immobilization mycelial pellet was selected as research object which was immobilized with lignin-degrading fungus G-13 and cellulose-degrading bacteria X10-1-2.Treating the papermaking wastewater,heavy metal wastewater and dye wastewater,and comparing with single fungal pellets in the performance of wastewater treatment.The results indicated that the immobilization mycelial pellet was better than the single fungal pellets in the treatment of wastewater,adsorption rate of the immobilization mycelial pellet was 10%,15% and 20% more than the single fungal pellets.It indicated that the immobilization mycelial pellet not only can be used in the treatment of papermaking wastewater,but also can be used for the treatment of dye wastewater and heavy metal wastewater.The immobilization mycelial pellet still has good degradation effect on papermaking wastewater in the high temperature environment.The degradation rates of lignin,cellulose and hemicellulose were 32.86%,36.09% and 37.91% of the immobilization mycelial pellet at 40 °C,It indicated that the immobilization mycelial pellet still has good degradation effect on papermaking wastewater in the high temperature environment.It indicated that the immobilization mycelial pellet can still maintain a high activity and has good effect on the treatment of three kinds wastewater in alkaline conditions.In summary,the strain G-13 had lignin degradation enzyme ability,the mycelial pellet in best condition was white,smooth surface,uniform size and good elasticity,it can satisfy the need of practical.The immobilization mycelial pellet can combinated with strains which can produce different enzyme.It showed good treatment effect in lignin removal,decolorization,adsorption of heavy metal ions and showed good treatment effect,provides a new way for industrial wastewater treatment. |
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