The Growth Inhibitory Effect And Algicidal Mech Anism Research Of Two Isoquinoline Alkaloids On Microcystis Aeruginosa And Chlorella Pyrenoi Dosa

In recent years, algae blooms occur frequently in many eutrophic lakes and the important drinking water source of our country, in which Microcystis aeruginosa outbreak rate is higher. The release of algal toxins will not only harm to water plants and animals but also affect human health. Seeking environmental frieldly, efficient and cheap method in control of M. aeruginosa is one of the hot issues in the environmental field. This paper studied inhibitory effect and inhibitory mechanism of two isoquinoline alkaloids, Chelerythrine and Sanguinarine, against M. aeruginosa NIES-843 and C. pyrenoidosa, and the possible mechanisms were involved. The main results are as follows:The EC50 of Chelerythrine stress on the growth of M. aeruginosa NIES-843 was (30.62±1.32) ?g/L. The reaction center of Microcystiis photosynthetic system electron providing side was one of the sites of Chelerythrine effected, extracellular polysaccharide content, protein content, phycocyanin content, extracellular alkaline phosphatase of M. aeruginosa NIES-843 have been restrained effectively. Thus, Chelerythrine is one kind of good biological substances on the inhibition against M. aeruginosa NIES-843.The EC50 of Sanguinarine stress on the growth of M. aeruginosa NIES-843 was (63.84±2.74) ?g/L. Chlorophyll photoinduced fluorescence kinetics results showed that when Sanguinarine concentration reaches 40 ?g/L, M. aeruginosa NIES-843 PS ? providing side and the cervix were damaged. When Sanguinarine concentration were 40 and 80 ug/L, chlorophyll a content, extracellular polysaccharide content, protein content, phycocyanin content, extracellular alkaline phosphatase of M. aeruginosa NIES-843 were lower than the control. These results showed that Sanguinarine had strong inhibitory against the growth of M. aeruginosa NIES-843.The effect of Chelerythrine and Sanguinarine stress on C. pyrenoidosa was similar,the EC50for Chelerythrine and Sanguinarine against C. pyrenoidosa was (31.62±2.19) ?g/L and (190.55±4.47) ?g/L, respectively, but they only played an inhibitory effect on the cells, did not cause too much impact on cells of chlorella chlorophyll a content, light absorption and excitation energy transfer process and the primary reaction of photosynthesis photochemical reaction. Chelerythrine and Sanguinarine can not play a lasting inhibitory effect on the intracellular polysaccharide content and protein content.The combined toxicity of Chelerythrine and Sanguinarine against M. aeruginosa NIES-843 were studied. The toxicity index was TI= 0.618< 1, it indicated that Chelerythrine and Sanguinarine showed a synergistic effect on M. aeruginosa NIES-843. The providing side of M. aeruginosa NIES-843 PS ? were damaged in the treatment of D and E group. The extracellular polysaccharide content, protein content, phycocyanin content, extracellular alkaline phosphatase of M. aeruginosa NIES-843 have been significantly suppressed in these two treatment. These results suggest that the control of M. aeruginosa using Chelerythrine and Sanguinarine composite is better than single of them.