Extraction,Purification And Vitro Activity Of Polysaccharides From The Flower Of Tea

Tea flower is tea(Camellia sinensis)tree's reproductive organ,It has a long history to plant tea tree in China,the annual production of tea tree flower is also very high,Many scholars have confirmed that a variety of physiological functions of polysaccharides,few studies focus on the polysaccharide of tea flower(TFPS),and it has been known that plant polysaccharides have a lot of biological activity.This article start from the extraction and purification of tea tree flower polysaccharide(TFPS),then study the physical and chemical properties,immune activity in vitro and antitumor activity in vitro of TFPS components.1.The extraction and purification,and the physical and chemical properties of TFPSThe optimum extraction parameters of TFPS were obtained as follows:extracting temperature 96?,extracting time 230 min,ratio of water to raw material 25 mL/g,Repeated twice.Then after filtered by 0.1?m,tea flower polysaccharide extraction was divided into two fractions,which were retentate liquid(TFPS-1)and permeate liquid(TFPS-2).The recovery rate were 5.82%and 0.63%.The retentate liquid and permeate liquid of TFPS were purified by DEAE-cellulose anion-exchange column chromatography,Six fractions were obtained(TFPS-1-1.TFPS-1-2,TFPS-1-3,TFPS-2-1,TFPS-2-2,TFPS-2-3).While TFPS-1-1 and TFPS-2-1 fractions were ignored because of few of them.Basic physicochemical characterization of TFPS-1,TFPS-2,TFPS-1-2,TFPS-1-3,TFPS-2-2,TFPS-2-3 were determined by methods of physics and chemistry and primary structure of TFPS-1-3 was speculated by NMR.2.The immune activity in vitro of TFPSIn this part,We study the immune activity in vitro of TFPS-1,TFPS-2,TFPS-1-2,TFPS-1-3,TFPS-2-2,TFPS-2-3,The research results show that,TFPS can accelerate Mouse Peritoneal Macrophages RAW264.7 value-added,promote the activity of acid phosphatase,promote phagocytosis neutral red,promote the release of NO?TNF-??IFN-??IL-1?,TFPS-1 and TFPS-2-2 has obvious immune activity in vitro.3.The antitumor activity in vitro of TFPSIn this part,We study the antitumor activity in vitro of TFPS-1,TFPS-2,TFPS-1-2,TFPS-1-3,TFPS-2-2,TFPS-2-3,The antitumor activity in vitro including:the influence of TFPS components on BGC survival rate,The influence of TFPS components on Caco-2 survival rate,The influence of TFPS components on HepG2 survival rate.Experimental results show that TFPS-1 has certain inhibition for stomach and colon cancer,TFPS-2 has certain inhibition for colon cancer,and TFPS have certain dose dependent inhibition on tumor cells,increase the processing time each component can show more value-added control capability.