Titanium Dioxide Nantubes And Its Surface Polydopamine Loaded With Copper Coating Modification

Blood contact materials required good blood compatibility.Although there had been a lot of researches turned to improve the hemocompatibility of blood contact materials,while more researches also are needed for the long-term aplication of blood contact materials.Titanium dioxide nanotubes arrays(TNT)could be used as drug carriers,and the release of drugs can be effectivelly controlled by TNT.In addition,studies had been shown that TNT could regulate verious cells adhesion,proliferation and differentiation behavior by regulating its diameter?In this study,the growth behavior of smooth muscle cells and blood compatibility on the different crystal forms TNT were studied by adjusting the the heat treatment temperature.In order to further improve the blood compatibility of TNT,in this paper,three kind of polydopamine coatings were deposited on TNT surface,and then the copper ion was fixed on the polydopamine coatings surfaces.This modified coatings could catalytic NO release through the copper,and the NO could further improve the biocompatibility of the material.The highly ordered TNT was prepared on the surface of titanium with the mothed of anodic oxidation technology.After the different heat treatment temperature(300?,450?,600?),the diameter structure,surface morphology,pipe diameter and tube wall of TNT were not changed obviously.The results of XRD showed that TNT annealed at 300? is anatase crystalline.After annealed at 450?,TNT appeared anatase and rutile two crystal forms,and the content of anatase crystal structure was higher.After annealied at 600?,the structure of TNT was also had both anatase and rutile two types,and the content of rutile crystal structure is higher.Subsequently,platelet adhesion and smooth muscle cell growth behavior was ecaluated,and the results indicated that the TNT film with anatase crystal structure obtained by heat treated at 300? could effectively inhibit the proliferation of smooth muscle cells.The TNT film with 600? treatment could effectively reduce the number of the adhered platelet.In addition,the polydopamine coatings with copper loaded was constructed on the surface of TNT,catalytic capacity of the surfaces was further confirmed using griess reagant and chemiluminescence.The hemocompatibility and cell compatibility of the coatings were studied.Three different TNT-based copper-loaded polydopaminecoatings were constructed by three different dopamine treatment times.Through the surface morphology detection of the coatings,it could be found that the inner diameter of TNT obtained by anodic oxidation is about 100nm,and the inner diameter is reduced to about 80nm after dopamine treatment.Through the oating detection of in vitro catalytic release of NO found that copper coatings could catalyze the release of NO from SNAP.In vitro study of endothelial cells and smooth muscle cells growth behavior could be found that copper-containing groups couldpromote endothelial cells,inhibit the proliferation of smooth muscle cells and inhibit platelet adhesion via catalyze the release of NO from SNAP.