Study On Zein Nanofiber And Calcium Phosphate Composite Material

Calcium phosphate cement(CPC)is an ideal artificial bone material used widely in hard tissue regeneration due to its excellent physical and chemical performance.However,the mechanical strength of CPC is similar to the cancellous bone as a brittle material,due to the poor flexual strength.Furthermore,CPCs are not able to match the high rate of new bone tissue growth during initial implantation given its poor degradability and low biological compatibility.These reasons are leading to their limited clinical application.This topic will study the surface modification and matrix modification of CPC by electrospinning technique.To show the feasibility of the composite material for hard tissue regeneration,it was characterized by SEM,porosity,hydrophilicity,mechanical properties,FTIR spectroscopy,and in vitro cell culture where the composite material showed improved in comprehensive performance.To overcome the limitations of CPC in biological properties,we developed a self-made electrospinning auxiliary receiver to obtain a three dimensional membrane coating of porous zein/PLLA coaxial nanofibers on CPC surfaces.To show the feasibility of the composite material for hard tissue regeneration,it was characterized by SEM,porosity,hydrophilicity,mechanical properties,FTIR spectroscopy,and in vitro cell culture where the composite material showed improved induction of tissue regeneration.Using a self-made auxiliary receiver and based on electrostatic spinning technology and salting out method,we have achieved an innovative CPC surface modification.The synthesis of a macroporous nanofiber 3D coating on the surface of a non-conductive material has led to the successful preparation of a novel type of composite artificial bone material.The results demonstrated that no major adverse chemical and biologic reactions were observed when using the zein/PLLA nanofiber CPC composite.Indeed,the materials did not only preserve the excellent physical and chemical performance of pure CPC,but also led to the formation of a hydrophilic surface,better mechanical properties,better biocompatibility,and a higher density of viable cells.Experimental results showed that the composites provided an adequate environment for cell adhesion and proliferation,indicating that the 3D coating of porous zein/PLLA coaxial nanofiber membranes on CPC surfaces composite is a potential scaffold material for bone tissue engineering.To overcome the limitations of CPC in porosity and mechanical properties,we developed a compound system of zein/PLLA blend nanofibers reinforced CPC and gelatin water-solubility obtained porous CPC.Using an innovative synchronized composite mode of the spinning and admix by self-made electrospinning auxiliary receiver,to obtain the zein/PLLA blend nanofibers reinforced CPC composite materials.On the basis of 3wt% nanofibers reinforced CPC as optimal flexual strength,the gelatin mixed with the CPC and nanofibers powder was removed as the result of its water-solubility,and then the nanofibers reinforced porous material was obtained.To show the feasibility of the composite material for hard tissue regeneration,it was characterized by SEM,porosity,hydrophilicity,mechanical properties,and FTIR spectroscopy where the composite material showed improved both in porosity and mechanical properties.Using zein/PLLA blend nanofibers reinforced and gelatin water-solubility obtained porous method,we have achieved an innovative CPC matrix modification.The synthesis of nanofibers and CPC porous material has led to the successful preparation of a novel type of composite artificial bone material.The zein/PLLA blend nanofibers were uniformly dispersed in CPC matrix.The nanofibers and CPC was good combination.With increasing the nanofibers content,the porosity was decrease gradually,the hydrophilic and flexural strength tend to increase first and then decrease gradually.The 3wt% nanofibers content composite material had the best hydrophilic and flexural strength.On the basis of 3wt% nanofibers reinforced CPC,the gelatin mixed with the CPC and nanofibers powder was removed as the result of its water-solubility,and then the nanofibers reinforced porous material was obtained.With increasing the gelatin content,the porosity and hydrophilic were increase gradually,while the flexural strength tend to decrease gradually.Indeed,with increasing porosity,the flexural strength values tended to decrease gradually,while sitill better than pure CPC due to the effect of nanofibers reinforced.The results demonstrated that no major adverse chemical and biologic reactions were observed when using the zein/PLLA nanofiber CPC composite.Experimental results showed that the composites provided a CPC composite material with higher porosity and better flexural strength,indicating that the nanofibers reinforced CPC porous material is a potential scaffold material for bone tissue engineering.