| Wound dressing is used to prevent further harm and supply good environment in order to make wound healing.As we all know,wound are easily attacked by bacteria,so wound dressings should prevent bacteria infection.Most of the wound dressings can’t meet the requirements of antibacterial.In this work,thermoplastic polyurethane(TPU)electrospun nanofibers as base material,and three kinds of antibacterial surface were constructed on the TPU electrospun nanofibers in order to improve the antibacterial property of the TPU electrospun nanofibers.The structure,composition and antibacterial property of the modified surface of the TPU electrospun nanofibers were studied.The detailed investigations are listed as follows.Part Ⅰ : Single layer bactericidal surface were designed on the TPU electrospun nanofibers.Multi-walled carbon nanotube(MWCNT)was facilely in-situ anchored onto thermoplastic polyurethane(TPU)electrospun nanofibers(TPU/MWCNT)by ultrasonication assistance to achieve the bactericidal property of TPU/MWCNT.The main conclusions are listed as follows:(1)X-ray photoelectron spectroscopy(XPS),field emission scanning electron microscope(SEM)and color change revealed that MWCNT could be successfully tightly anchored onto the surface of TPU electrospun nanofibers.(2)TPU/MWCNT exhibited excellent antibacterial properties evaluated through the spread plate test and field emission scanning electron microscope(SEM).Part Ⅱ : In this article,double layer bactericidal surface were designed on the TPU electrospun nanofibers.Multi-walled carbon nanotube(MWCNT)modified with Vancomycin(Van)was synthesized via the chemical reaction of MWCNT’s inherent carboxyl group and Van’s amide group as antibacterial agent.Then,Van-MWCNT was facilely in-situ anchored onto thermoplastic polyurethane(TPU)electrospun nanofibers(TPU/Van-MWCNT)by ultrasonication assistance to achieve the bactericidal property of TPU/Van-MWCNT.The main conclusions are listed as follows:(1)The Fourier transform infrared spectroscopy(ATR-FTIR)and X-ray photoelectron spectroscopy(XPS)revealed that Van was reacted with MWCNT,indicating the successful modification of Van on MWCNT.(2)X-ray photoelectron spectroscopy(XPS),field emission scanning electron microscope(SEM)and color change revealed that Van-MWCNT could be successfully tightly anchored onto the surface of TPU electrospun nanofibers.(3)The minimum inhibitory concentration(MIC)of Staphylococcus aureus(S.aureus)for Van-MWCNT was 1~10 mg/mL and MWCNT was 25~50 mg/mL.Hence,Van-MWCNT exhibited better antibacterial property for S.aureus than MWCNT.(4)TPU/Van-MWCNT exhibited excellent double layer antibacterial properties evaluated through the spread plate test and field emission scanning electron microscope(SEM).Part Ⅲ : In this article,bactericidal and antifouling surface were designed on the TPU electrospun nanofibers.Poly(ethylene glycol)methacrylate(PEG)was chemically grafted onto TPU nanofibers through UV photo-graft polymerization to obtain TPU-g-PEG nanofibers,followed by the tight immobilization of CNT as bactericidal components onto the TPU-g-PEG nanofibers under ultrasonication assistance.The main conclusions are listed as follows:(1)X-ray diffraction(XRD),field emission scanning electron microscope(SEM),Drop Shape Analyzer(DSA)confirmed that the PEG has successfully tightly anchored onto the surface of TPU electrospun nanofibers.(2)X-ray diffraction(XRD),field emission scanning electron microscope(SEM),Drop Shape Analyzer(DSA)and color change confirmed that the CNT has successfully deposited on the surface of TPU-g-PEG electrospun nanofibers.(3)Red blood cell(RBC)adhesion and hemolysis ratio test confirmed that PEG has positive effect on the blood red cell adhesion and hemolysis and TPU-g-PEG/CNT nanofibers exhibited excellent hemocompatibility,including suppression of red blood cells adhesion,and the lower hemolysis ratios.Importantly.(4)TPU-g-PEG/CNT nanofibers exhibited excellent antibacterial properties andantifouling properties evaluated through the spread plate test and field emission scanning electron microscope(SEM). |
