Studies On The Hydrolysis Of Veal Bone And Its Antioxidative Activity Of Its Enzyme Content

China is a major producer of meat,and bone is an important by-product.With fresh cow leg bone as raw material,this paper measured its basic component.After made into powder by degreasing and grinding,the best condition of extracting protein under high heat and pressure was explored.Pepsin and trypsin were chosen to hydrolyze bone powder in a two-step mode,and the optimal dual-enzyme hydrolysis condintions of bone powder were explored for maximizing degree of hydrolysis.On this basis,the antioxidant activity of enzymatic hydrolysate under different hydrolysis time was studied.1?Components analysis of veal bone.Among the determination of veal bone,the basic ingredients are:water content of 62.3%;protein content of 12.7%;fat content of 8.7%;the total ash content of 15.2%.2?Determination of optimum pretreating conditions of veal bone powder.Pretreating time before the preparation of veal bone powder was discussed.Then the factors that affect the pretreating conditions of veal bone powder were studied by single factor experiment.The best experimental conditions are as follow.Veal bone powder was made by way of degreasing and drying after pretreating under 0.1 MPa,121?,When the veal bone powder was cooked for 6 hours under 0.1MPa,121? with solid-liquid ratio 1:6 and pH 5.The crude protein in bone soup was 15.298%.3?Determine optimum enzymatic conditions.after The powders of veal bone pretreated single factor experiments and orthogonal test were adopted to hydrolyze the powders.The optimal hydrolysis conditions of pepsin was:pH 2.0,temperature 32?,the substrate concentration 5%,enzyme concentration 4‰,the enzyme digestion 90min,and in this condition,the degree of hydrolysis is 0.144.On the basis of optimum pepsin hydrolysis conditions,trypsin was added to the second-stage hydrolysis,the optimal hydrolysis conditions of trypsin are as.follows:pH 7.5,temperature 50?,substrate concentrations%,enzyme concentration 6‰,the degree of hydrolysis is 21.1%.4?Study on antioxidation activity of hydrolysates.In the experiment of hydrolyzing bovine bone powder pepsin-trypsin step-by-step,the total reduction and radical scavenging rate were different under the different time.When hydrolying 3h,the total redudtion and radical scavenging rate have reached the maximum,30.5%and 71.4%respectively.