Effects Of Phytomedical Part,Compatibility,and Extraction Technology On The Species And Bioavailability Of Metal Complexation

Monolayer liposome was used as the biomembrane model,monolayer liposome-water system was adopted to simulate the distribution and absorption of trace metals complexation in extraction liquor and the cheym from the in vitro bionic digestion of phytomedicine in human body.Monolayer-liposome-and water-solubility were used for species definition and bioavailability assessment of trace metals complexation.To study the effects of phytomedicine parts,compatibility and extraction technologies on the speciation analysis,bioavailability of trace metals complexation.(1)A pre-treatment technology,in vitro bionic digestion was designed,according to:(a)chemical bionic stomach and intestine,(b)whole medical effective,and(c)methods in molecular medicine,using trace metals as indicator.The MLSMs content was an important and accurate criterion for metal bioavailability and risk assessment.Based on the concentration of MLSMs,the metal main absorption site was proposed.Significant influence of phytomedical part on the speciation analysis,bioavailability of trace metals complexation.The safe dosage and tolerable upper intake level of the stem and leaves of A.spinulosa and P.vittata for adults were proposed as 210.4 and 864.1 g/day for the stem of A.spinulosa;153.5 and 1208.8 g/day for the leaves of A.spinulosa;828.2 and 2067.1 for the stem of P.vittata;367.5 and 1977.3 g/day for the leaves of P.vittata,respectively.(2)The influence of gastrointestinal digestive enzymes and the compatibility of phytomedicine on metal bioaccessibility and bioavailability were initially discussed to understand the action of gastrointestinal digestive enzymes and phytomedicine compatibility.The best combination ratios of Prunus persica Batsch(A)and Carthamus tinctorus(B)was 1A:1B.The safe dosage is calculated as 421.0 g/day for males and 500.0 g/day for females,respectively.The maximum value is 2368.4g/day for males and females.(3)The influence of four extraction technologies,including water decocting,ethanol extracting and semi-bionics(simulating the acidity of gastric juice or intestinal juice),the extracted percentage,species and bioavailability of iron and manganese in Fujian characteristic of phytomedicine(Herba Anoectochili and Radix Morindae Officinali)was studied.The results indicated that:(a)the extracted percentages of iron and manganese were the highest in the extraction liquor with acid,while the least were in ethanol liquor;(b)the concentrations of monolayer-liposome-solubility of iron and manganese were also the highest in the liquor extracted by acid,so its pharmaceutical effect was the best;(c)metal content in monolayer liposome could be used to assess the bioavailability and pharmacological effects in phytomedicine.Metal species and bioavailability were greatly affected by extraction procedures and such influences should be taken into account for the optimum dose design,bioavailability and risk assessment about trace metals in phytomedicine.Based on the metal effectiveness and safety,both a standard to establish phytomedical dose and a new method to evaluation extraction technologies were suggested.