Study And Application Of Metal-organic Frameworks As Peroxidase Mimic

Metal-organic frameworks(MOFs),which are constructed by organic ligand and metal ion with self-assembly,are crystal materials of periodic network structure,and exhibit unprecedentedly large and permanent inner porosity,large surface area,tunable pore size and functionality,structural diversity and excellent thermal stability.MOFs have shown promising applications in adsorption,catalysis and sensing.In this paper,we prepared several kinds of metal-organic frameworks using simple synthesis method,investigated their of peroxide mimic properties and applied to the analysis of several kinds of target.This paper contains five chapters.In the first chapter,a briefly reviewed about MOFs was made,including the concept,development status,the classification,etc.Then,their excellent properties,synthesis methods and applications in the field of separation,catalysis,sensors and so on were introduced.In the second chapter,Pt@MIL-100(Fe)was prepared through a simple ascorbic acid reduction method with uniform distribution nanoparticles,then facilitate the TMB-H2O2 chromogenic system research the differences of catalytic activity between MIL-100(Fe)and Pt@MIL-100(Fe).Kinetic analysis measures indicated that the catalytic behavior was consistent with typical Michaelis-Menten kinetic.Here,The liner detecting range of uric acid was 1.2 pg/mL-12.0 ?g/mL with a detection limit of 0.4 ?g/mL(S/N=3).In the third chapter,using a simple hydrothermal method was synthesized NH2-MIL-101(Fe)with FeCl3 · 6H2O and aminoterephthalic acid as the reactants.By adding in acetic acid or sodium acetate to adjust pH value of reaction liquid obtained the different length and morphology of crystals,and explore the catalysis activity.The results indicated that longer crystal have small influence on catalytic activity,while shorter and wider crystal could increase the performance.For the first time,We demonstrated this materials possess intrinsic peroxidase mimic activity,and applied it to test the hypoxanthine in serum.The liner detecting range was from 0.4 ?g/mL to 4.0 ?g/mL and the detection limit was 0.013 ?g/mL(S/N=3).In the fourth chapter,using the simple synthesis method to get three kinds of MOFs materials based on copper.The morphology of these materials is different,respectively of octahedron,tetrahedron and nanosheet.Then we applied TMB-H2O2 chromogenic system to studied them catalysis activity,found that nanosheet>tetrahedron>octahedron,due to two-dimensional structure can exposure the active site more easily.Finally,we used this material to detect glucose in serum,the system exhibited a good linear relationship with the concentration of glucose in the range of 0.05 to 0.45 mM with a detection limit of 0.017 mM(S/N=3).