Design,Synthesis And Biomimetic Applications Of Two-photon Fluorescence Probes Based On Oxyanthracene And Naphthalene Derivatives

Fluorescent probes as a kind of high sensitivity,low detection limit,good selectivity,low cost analysis testing technology are constantly applied to biology,chemistry,medicine and environmental testing field.At present,although there has been designed and developed many fluorescent probes to detect various ions,active substances,enzymes,most of these probes use one-photon excitation which limits applications in biological systems.Compared with one-photon technology,two-photon imaging technology as a new imaging technology has some advantages such as using infrared photons as the excitating wavelength,the larger space resolution,deep tissue penetration and long fluorescent observation time,etc.In order to imaging with two-photon microscope,two-photon probes must have greatly two-photon absorption cross sections.So the development of large two-photon absorption cross sections are of great significance to the fluorescent probes,and the research of two-photon fluorescence probes has become a hot topic in the study of the international community.This paper use xanthene and naphthalene as precursor to detect nitroreductase,Pd(0),hydrazine respectively.Firstly,we used xanthene fluorescent dye GCTPOC which has a great two-photon absorption cross section to design a new type of nitratereductase fluorescence probe.The probe used nitro benzyl bromide as the recognition site of nitroreductase.We tested the probe with spectral performance and cytotoxicity.It was found that the response of NTR was fast,the detection limit was low and it had little cytotoxicity,which was suitable for use in biological system.In addition,we used the probe to detect NTR in hypoxic tumor cells and tumor tissue successfully.We expect that the probe will suited for the detection and treatment of neoplastic diseases.Secondly,the zero-valent palladium fluorescent probe was synthesized by using the two-photon fluorescent dye GCTPOC as the matrix.As the hydroxyl was protected,the fluorescence was turn-off.After adding zero-valent palladium,light was turn-on,making the fluorescence enhancement.The probe responded quickly to zero-valent palladium and fluorescence was enhanced to maximum within 6 minutes.The probe detected cells and tissues of zero-valent palladium and displayed penetration up to a depth of 170 ?m.Thirdly,we used benzothiazole-2-acetonitrile as a new type of identification sites of hydrazine firstly and naphthalene as the fluorescent precursor.The probe had no fluorescence,after the addition of hydrazine,the light became turn-on.The probe had good light stability and good selectivity to the target,so it was suitable for applied to detection of hydrazine in cells and tissues.In addition,we had effectively tested the hydrazine in the environment with this probe.There was great potential in detection of hydrazine in environment and vivo.