Preparation And Characterization Evalutation Of Molecularly Imprinted Polymer Microspheres With Three Dimensionally Ordered Macroporous Materials

Ordered macroporous microspheres material is a porous material,with regular,uniform,the same channel as well as the large surface areas and special optical properties,so it has good prospects at Chromatographic stationary phases Photonic crystals,catalysts and such areas as drug delivery carrier and so on.Molecular Imprinting technique is aimed at a specific target moleculedesign and synthesis of its highrecognitionof molecularly imprinted polymers(MIP).Molecular imprinting polymerpre pared has a wide range of applications in chromatography,solid phase extraction,sensor.This paper we comine the preparation of ordered macroporous materials technology and molecular imprinting technique,study on the preparation method of three dimensional ordered macroporous molecularly imprinted microspheres,the microspheres has recognition properties of moleculary imprinted materials and permeability of ordered porous materials,overcoming the defect of traditional molecular imprinted polymer with lower identifical dynamics rate,low efficiency,therefore it is an ideal chromatographic material.This paper consists of three parts.Part one:Studing the preparation technology of ordered macroporous microsphere,setting SiO2 and polystyrene or its copolymers as colloidal crystal templates to fabricate three dimensional ordered porous microspheres,and their preparation conditions such as assembling colloidal crystal template,the composition of precusor solution,permeability and polymerization conditions were discussed.Results showed that when mixing speed was 300r,assembling temperature was 70 ?,monodisperse of colloidal crystals prepared well,colloidal particles was in face-centered cubic packing.When selecting modified western blotting system formula,PEG was directly sealed after penetration and osmosising twice,microspheres prepared was uniform in diameter,arranged in order and performed the less defects.We use sol-gel method and total deposition to prepare three dimensional ordered porous microspheres when setting polystyrene or its copolymers as colloidal crystal templates.Studies have found that using V(ethanol:silicon source)=2:1,macro-porous microspheres prepared by sol-gel method arranged uniform pore size,high order;as well,when solid phase volume ratio of silica was 7.3%,macroporous microspheres prepared performance fewer defects.Part two:using quinine as imprinted molecules,methacrylic acid(MAA)as functinal monomer,ethylene glycol dimethyl acrylate ester(EDMA)as the cross-linking agent,hydroxyethyl methacrylate(HEMA)and silicon-based KH570 as a secondary monomer,SiO2 microspheres as colloidal crystal template,we prepare the dinorganic-organic hybrid macroporous polymeric imprinted microspheres successfully by combining colloidal crystal template method and molecular imprinting technique.As for the macroporous materials,whose structure was stable,mechanical intensity was good and the big hole arranged rule,it copied the FCC structure of the colloidal crystal template.Compared to conventional body imprinted polymers,it.had higher swelling properties and high porosity than bulk polymer.Dynamics model and selective adsorption showed that MIPC and had faster absorption rate and specific performance relative to the bulk polymer.Part three:We prepared surface grafting double bond of ordered macroporous SiO2 microspheres using KH570 modified silica deposition together with PS,setting quinine as imprinted molecule,MAA as functional monomers,EDMA as crosslinker,ABVN as initiator,acetonitrile as solvent,besides,imprinting in macroporous Silicon microspheres hole surface,we prepared surface grafting 3DOM macroporous SiO2 molecular Imprinting microspheres successfully,as well,SEM,BET,and IR,TG were studied.Studies showed that 3DOM MIP microspheres had ordered pore structure,higher porosity.