Research On The Separation And Analysis Methods Of Carbazochrome Sodium Sulfonate

Carbazochrome sodium sulfonate(CSS)as a new generation of hemostatic drug,has been more and more used in clinical treatment.Polymer nanoparticles adsorbent(PNPs)and carbazochrome sodium sulfonate molecularly imprinted polymer nanoparticles(CSS-MIP NPs)were prepared with CSS as target analyte,and used for separation and enrichment of CSS.This paper also studied the interaction between silver nanoparticles and CSS with the resonance light scattering spectrometry,and a new method for determination of CSS was established.This paper mainly consists of the following four parts:In chapter 1,the properties and the analytical methods of CSS were summarized.This paper introduced the principle and applications of molecular imprinting technology.The characteristics and application of the nanomaterials were briefly discussed.In chapter 2,the PNPs were prepared with[2-(methacryloyloxy)ethyl]trimethylammonium chloride(DMC)as functional monomer and ethylene glycol dimethacrylate(EDMA)as crosslinking agent,and were characterized by Fourier transform infrared spectroscopy(FT-IR)and transmission electron microscopy(TEM).The synthesis,adsorption and elution conditions were optimized.The adsorption thermodynamics and kinetics of PNPs for CSS were studied.The results show that adsorption capacity reached maximum after 4 min at pH 5,and the maximum adsorption capacity was 210.3 mg/g.The quantitative desorption was achieved after 5 min by using 10.0 mL 2 mol/L HCl solution as eluent.PNPs were regenerated for 10 times without significant decrease in absorption capacity.The enrichment factor of PNPs was 80.In chapter 3,the CSS-MIP NPs were synthesized with CSS as template molecule,4-vinylpyridine(4-VP)and acrylamide(AM)as bio-functional monomer and EDMA as cross-linking agent.The CSS-MIP NPs were characterized by FT-IR and TEM,and the specific surface area was measured.Experimental conditions for effective adsorption of CSS were optimized with respect to different experimental parameters using static procedures in detail.The results show that CSS-MIP NPs had high adsorption capacity for CSS.The maximum adsorption capacity of CSS-MIP NPs was 280.5 mg/g.The adsorption equilibrium only needed 5 minutes.CSS-MIP NPs have good adsorption selectivity and reusability for CSS.In chapter 4,silver nanoparticles were prepared by chemical reduction method.The interaction between silver nanoparticles and CSS was studied,and a novel method was developed to determine trace CSS by resonance light scattering(RLS).The experiment conditions,such as reaction time,concentration of NaOH,ion strength and interfering ions,were investigated.The linear range was 1.0×10-7-1.5×10-5 mol/L,the detection limit was 6.0×10-8 mol/L,and the recovery was between 97.44%and 105.9%.The method was successfully applied for the determination of CSS in real samples.